Development and in vitro assay of oxidative stress modifying formulations for wound healing promotion
| Autor: | Nicolas Atrux-Tallau, Fabrice Pirot, Françoise Falson, Valérie Bertholle, Marek Haftek, Karine Padois, Sylvie Callejon, Camille Migdal, Alain Denis, Marlène Chavagnac-Bonneville |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Keratinocytes
Vascular Endothelial Growth Factor A Chronic wound medicine.medical_specialty Cell signaling Alginates Inflammation Dermatology medicine.disease_cause In vivo medicine Humans Cells Cultured chemistry.chemical_classification Chitosan Wound Healing Reactive oxygen species business.industry Hydrogen Peroxide Microspheres Surgery Cell biology Oxidative Stress medicine.anatomical_structure chemistry medicine.symptom Cell Migration Assays Reactive Oxygen Species business Keratinocyte Wound healing Oxidation-Reduction Oxidative stress |
| Zdroj: | European Journal of Dermatology. 21:52-62 |
| ISSN: | 1952-4013 1167-1122 |
| DOI: | 10.1684/ejd.2011.1270 |
| Popis: | Often presented as metabolism byproducts, reactive oxygen species are linked to detrimental effects such as chronic wound, mutagenesis, cancer and skin ageing. However, recent in vitro and in vivo observations suggest that ROS, and mainly hydrogen peroxide, interfere with cell signaling acting like second messenger and inducing adaptive responses. This is particularly observed in skin wound healing where cells are exposed to H₂O₂ following injury. In this study, we developed and characterized an innovative formulation producing H₂O₂ at low concentrations, in order to mimic physiological inflammation phase. Then, this pro-oxidative formulation (CAM-GOx) was assayed in vitro on keratinocytes cell culture, compared to the blank formulation (CAM) and the anti-oxidative formulation (CAM-CAT) to assess whether oxidative stress was implied or not in cellular responses. |
| Databáze: | OpenAIRE |
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