Rhizobium altiplani sp. nov., isolated from effective nodules on Mimosa pudica growing in untypically alkaline soil in central Brazil
| Autor: | Jean Luiz Simões-Araújo, Marta Maluk, Jerri Édson Zilli, Pietro P. M. Iannetta, Veronica Massena Reis, A. C. Baraúna, Fábio Bueno dos Reis Junior, Euan K. James, Silvia Regina Goi, Luc Felicianus Marie Rouws |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Mimosa Sequence analysis Microbiology Soil 03 medical and health sciences Symbiosis Nitrogen Fixation RNA Ribosomal 16S Botany Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Mimosa pudica Fatty Acids Nucleic Acid Hybridization food and beverages Sequence Analysis DNA General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification rpoB 16S ribosomal RNA Bacterial Typing Techniques Housekeeping gene 030104 developmental biology Genes Bacterial Nitrogen fixation bacteria Rhizobium Root Nodules Plant Brazil |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 66:4118-4124 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001322 |
| Popis: | Root nodule bacteria were isolated from nodules on Mimosa pudica L. growing in neutral-alkaline soils from the Distrito Federal in central Brazil. The 16S rRNA gene sequence analysis of 10 strains placed them into the genus Rhizobium with the closest neighbouring species (each with 99 % similarity) being Rhizobium grahamii, Rhizobium cauense, Rhizobium mesoamericanum and Rhizobium tibeticum. This high similarity, however, was not confirmed by multi-locus sequence analysis (MLSA) using three housekeeping genes (recA, glnII and rpoB), which revealed R. mesoamericanum CCGE 501T to be the closest type strain (92 % sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with majority being C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1ω7c/C18 : 1ω6c)], DNA G+C content (57.6 mol%), and carbon compound utilization patterns supported the placement of the novel strains in the genus Rhizobium. Results of average nucleotide identity (ANI) differentiated the novel strains from the closest species of the genus Rhizobium, R. mesoamericanum, R. grahamii and R. tibeticum with 89.0, 88.1 and 87.8 % similarity, respectively. The symbiotic genes essential for nodulation (nodC) and nitrogen fixation (nifH) were most similar (99-100 %) to those of R. mesoamericanum, another Mimosa-nodulating species. Based on the current data, these 10 strains represent a novel species of the genus Rhizobium for which the name Rhizobium altiplani sp. nov. is proposed. The type strain is BR 10423T (=HAMBI 3664T). |
| Databáze: | OpenAIRE |
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