Evaluation of paraoxonase, arylesterase, and homocysteine thiolactonase activities in patients with diabetes and incipient diabetes nephropathy
| Autor: | Sibel Soylemez, Ayşe Banu Çayci, Durmuş Ayan, Mehmet Senes, Nezaket Eren, Yuksel Altuntas, Feyza Yener Ozturk |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Homocysteine paraoksonaza homocysteine thiolactonase 030204 cardiovascular system & hematology AutoAnalyzer homocistein tiolaktonaza Nephropathy lcsh:Biochemistry Arylesterase Diabetic nephropathy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Diabetes mellitus Internal medicine Medicine lcsh:QD415-436 arilesteraza Original Paper biology arylesterase business.industry Paraoxonase medicine.disease paraoxonase Enzyme assay 030104 developmental biology Endocrinology chemistry biology.protein business |
| Zdroj: | Journal of Medical Biochemistry, Vol 38, Iss 4, Pp 481-488 (2019) Journal of Medical Biochemistry |
| ISSN: | 1452-8266 |
| DOI: | 10.2478/jomb-2019-0014 |
| Popis: | The aim of this study is to examine the relationship among the changes in activities of paraoxonase (PON), arylesterase (ARE) and homocysteine thiolactonase (HTLase) enzyme having antioxidant properties and the development of diabetic nephropathy (DN), one of the most common complications of diabetes.Normoalbuminuric type-2 diabetic patients (Group II, n=100), microalbuminuric type 2 diabetic patients (Group III, n=100) and the control group (Group I, n=100) were included in the study. The age and gender of the patient groups matched with the age and gender of the control group. HTLase, PON and ARE enzyme activities were measured by the spectrophotometric method using a g-thiobutyrinolactone, paraoxon, and phenylacetate substrates respectively. In this study, an autoanalyzer application was developed in order to measure HTLase enzyme activity for the first time.Serum HTLase, ARE and PON activities of Group III and Group II were significantly low compared to HTLase, ARE and PON results of Group I (p0.05).Based on our results, PON, ARE and HTLase enzyme activities were found to be decreased due to the increase in the degree of DN.Cilj ovog istraživanja je da se ispita odnos između promena u aktivnostima paraoksonaze (PON), arilesteraze (ARE) i homocistein tiolaktonaze (HTLase) sa antioksidativnim svojstvima i razvojem dijabetičke nefropatije (DN), jednog od najčešćih komplikacija dijabetesa.U studiju su uključeni bolesnici sa dijabetesom tipa 2 u stanju normoalbuminurije (grupa II, n = 100), pacijenti sa dijabetesom tipa 2, mikroalbuminurnog tipa (III grupa, n = 100) i kontrolna grupa (grupa I, n = 100). Pol pacijenata i njihova starosna dob su se podudarali sa starošću i polom kontrolne grupe. Aktivnost enzima HTLase, PON i ARE je merena spektrofotometrijskom metodom uz korišćenje supstrata γ-tiobutirinolaktona, paraoksona i fenilacetata. Ovim istraživanjem je razvijena primena autoanalizatora kako bi se prvi put merila aktivnost enzima HTLase.Aktivnosti serumske HTLaze, ARE i PON kod grupe III i grupe II bile su značajno niske u poređenju sa rezultatima HTLaze, ARE i PON kod grupe I (p0,05).Na osnovu naših rezultata utvrđeno je da su aktivnosti enzima PON, ARE i HTLase smanjene zbog povećanja stepena DN. |
| Databáze: | OpenAIRE |
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