Cold-priming of chloroplast ROS signalling is developmentally regulated and is locally controlled at the thylakoid membrane
| Autor: | Margarete Baier, Jörn van Buer, Andreas Prescher |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell signaling Chloroplasts Arabidopsis 500 Naturwissenschaften und Mathematik::570 Biowissenschaften Biologie::571 Physiologie und verwandte Themen lcsh:Medicine Biology Thylakoids Article plant molecular biology 03 medical and health sciences 0302 clinical medicine Ascorbate Peroxidases Transcription (biology) Gene Expression Regulation Plant Cold acclimation plant signalling lcsh:Science Regulation of gene expression Multidisciplinary Arabidopsis Proteins lcsh:R biology.organism_classification Cell biology Chloroplast Plant Leaves 030104 developmental biology Peroxidases Thylakoid biology.protein lcsh:Q abiotic Reactive Oxygen Species 030217 neurology & neurosurgery Peroxidase Signal Transduction |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) |
| Popis: | 24 h exposure to 4 °C primes Arabidopsis thaliana in the pre-bolting rosette stage for several days against full cold activation of the ROS responsive genes ZAT10 and BAP1 and causes stronger cold-induction of pleiotropically stress-regulated genes. Transient over-expression of thylakoid ascorbate peroxidase (tAPX) at 20 °C mimicked and tAPX transcript silencing antagonized cold-priming of ZAT10 expression. The tAPX effect could not be replaced by over-expression of stromal ascorbate peroxidase (sAPX) demonstrating that priming is specific to regulation of tAPX availability and, consequently, regulated locally at the thylakoid membrane. Arabidopsis acquired cold primability in the early rosette stage between 2 and 4 weeks. During further rosette development, primability was widely maintained in the oldest leaves. Later formed and later maturing leaves were not primable demonstrating that priming is stronger regulated with plant age than with leaf age. In 4-week-old plants, which were strongest primable, the memory was fully erasable and lost seven days after priming. In summary, we conclude that cold-priming of chloroplast-to-nucleus ROS signalling by transient post-stress induction of tAPX transcription is a strategy to modify cell signalling for some time without affecting the alertness for activation of cold acclimation responses. |
| Databáze: | OpenAIRE |
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