New Qualitative Detection Methods of Genetically Modified Potatoes
| Autor: | Takahiro, Watanabe, Hideo, Kuribara, Takashi, Mishima, Hiroyuki, Kikuchi, Takashi, Kodama, Satoshi, Futo, Kikuko, Kasama, Akie, Toyota, Masanori, Nouno, Ayako, Saita, Kunihiko, Takahashi, Akihiro, Hino, Hiroshi, Akiyama, Tamio, Maitani, Misao, Kubo |
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| Rok vydání: | 2004 |
| Předmět: |
UTP-Glucose-1-Phosphate Uridylyltransferase
Bacterial Toxins DNA Recombinant Pharmaceutical Science Biology Polymerase Chain Reaction Sensitivity and Specificity law.invention Hemolysin Proteins Bacterial Proteins Japan Test material law Screening method Gene Polymerase chain reaction DNA Primers Solanum tuberosum Pharmacology Genetics Bacillus thuringiensis Toxins fungi food and beverages General Medicine Plants Genetically Modified Genetically modified organism Endotoxins genomic DNA Recombinant DNA Primer (molecular biology) |
| Zdroj: | Biological and Pharmaceutical Bulletin. 27:1333-1339 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.27.1333 |
| Popis: | In Japan, 8 lines of genetically modified (GM) potato (2 lines of NewLeaf® potato; NL, 3 lines of NewLeaf Plus® potato; NLP, and 3 lines of NewLeaf Y® potato; NLY) have already been authorized as safe for use in foods and feeds. We have developed polymerase chain reaction (PCR) methods for the qualitative detection of the GM potatoes for the screening and the identification of NL, NLP and NLY. The gene encoding uridine diphosphate (UDP)-glucose pyrophosphorylase (UGPase) was used as a taxon specific gene. We designed the primer pair to detect the cryIIIA genes as a screening method for GM potatoes because the gene should be inserted in all 8 lines of the GM potatoes. For identification of NL, NLP and NLY, we further designed three specific primer pairs for the different recombinant DNAs (r-DNA) specifically introduced into NL, NLP, or NLY. In addition, to identify the 3 lines of NLY that have been introduced with the same r-DNA, the three line-specific primer pairs for the border sequence between the r-DNA and genomic DNA of NLY 3 lines were designed. Six lines of GM potato used as the test material were specifically identified using the each primer pair under the same PCR condition. The detection limits of all the GM potatoes should be approximately 0.1%. Furthermore, the specificity and reproducibility of the methods were confirmed in a six-laboratory collaborative study. |
| Databáze: | OpenAIRE |
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