Effect of estradiol and clomiphene citrate on Erk activation in breast cancer cells
| Autor: | Sami Aifa, Ikram Jemel-Oualha, Bochra Hakim, Abdelhamid Beji, Jihene Elloumi-Mseddi |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
MAPK/ERK pathway
medicine.medical_specialty Cell Survival MAP Kinase Signaling System Apoptosis Breast Neoplasms Biology Biochemistry Clomiphene Internal medicine medicine Humans Viability assay Phosphorylation Protein kinase A Molecular Biology Cell Proliferation Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Estradiol Kinase Cell growth Cell Biology Endocrinology Cancer research MCF-7 Cells Female Signal transduction |
| Zdroj: | Journal of receptor and signal transduction research. 35(2) |
| ISSN: | 1532-4281 |
| Popis: | The mitogen-activated protein kinase (MAPK) signaling pathway plays key roles in the transmission of proliferative signals in normal and dysregulated cells. Nevertheless, some studies have shown that activation of the extracellular regulated kinases 1/2 (Erk1/2) is involved in apoptosis. In this study, we evaluate the effect of two fertilizing drugs, clomiphene citrate and estradiol, on the activation of Erk1/2 and the viability of two breast cancer cell lines, MCF-7 (hormone dependent) and BT20 (hormone independent).We show that both drugs induce Erk1/2 phosphorylation in MCF-7 and BT20 cells despite their opposite effect on cell viability. In fact, clomiphene citrate is significantly proapoptotic while estradiol promotes cell proliferation. The fact that phospho-Erk1/2 is a common element to both mechanisms suggests that specific factors deciding between proliferation and apoptosis must be operative downstream of this signaling pathway. |
| Databáze: | OpenAIRE |
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