New observations on gametogenic development and reproductive experimental tools to support seed yield improvement in cowpea [Vigna unguiculata (L.) Walp.]
| Autor: | Susan D. Johnson, Jean-Philippe Vielle-Calzada, Anna M. G. Koltunow, Rigel Salinas-Gamboa, Nidia Sánchez-León |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Megagametogenesis Megasporogenesis Stamen Plant Science medicine.disease_cause 01 natural sciences Microgametogenesis Vigna 03 medical and health sciences Pollen Botany medicine Ovule Gametogenesis Gametogenesis Plant Reproductive calendar biology food and beverages Cell Differentiation Cell Biology biology.organism_classification Whole-mount immunolocalization 030104 developmental biology Agronomy Fertilization Cowpea Original Article Megaspore mother cell Megaspore 010606 plant biology & botany |
| Zdroj: | Plant Reproduction |
| ISSN: | 2194-7961 2194-7953 |
| Popis: | Key message Cowpea reproductive tools. Abstract Vigna unguiculata L. Walp. (cowpea) is recognized as a major legume food crop in Africa, but seed yields remain low in most varieties adapted to local conditions. The development of hybrid cowpea seed that could be saved after each generation, enabling significant yield increases, will require manipulation of reproductive development from a sexual to an asexual mode. To develop new technologies that could support the biotechnological manipulation of reproductive development in cowpea, we examined gametogenesis and seed formation in two transformable, African-adapted, day-length-insensitive varieties. Here, we show that these two varieties exhibit distinct morphological and phenological traits but share a common developmental sequence in terms of ovule formation and gametogenesis. We present a reproductive calendar that allows prediction of male and female gametogenesis on the basis of sporophytic parameters related to floral bud size and reproductive organ development, determining that gametogenesis occurs more rapidly in the anther than in the ovule. We also show that the mode of megagametogenesis is of the Polygonum-type and not Oenothera-type, as previously reported. Finally, we developed a whole-mount immunolocalization protocol and applied it to detect meiotic proteins in the cowpea megaspore mother cell, opening opportunities for comparing the dynamics of protein localization during male and female meiosis, as well as other reproductive events in this emerging legume model system. Electronic supplementary material The online version of this article (doi:10.1007/s00497-015-0273-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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