Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams
| Autor: | Renato L. Santos, E.F. Nascimento, Olindo Assis Martins-Filho, Cláudia Emanuela Viana Rocha, Luciana F. Costa, Kessler W. J. Fonseca, Renata Mondêgo de Oliveira, Luize Néli Nunes Garcia, Humberto M. Brandão, Tatiane A. Paixão, Jade R. D. Farias, Márcio Sobreira Silva Araújo, Gabriela G. Neves, Everton de Lima Romão, Priscilla Parreira Rocha Gomes, Juliana Pinto da Silva Mol, Teane M. A. Silva, Gustavo C. Teixeira, Andréa R. F. Maia, Ana Patrícia C. Silva, Auricélio A. Macêdo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Brucella ovis Alginates Injections Subcutaneous Science Brucella Vaccine Sheep Diseases Capsules Semen Brucella Urine Brucellosis Bacterial Proteins Glucuronic Acid medicine Animals Ovis Sheep Domestic Sheep Multidisciplinary biology Hexuronic Acids Wild type medicine.disease biology.organism_classification Virology Blood Immunization Medicine ATP-Binding Cassette Transporters Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 8, p e0136865 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|