Mass kinetics of apolipoprotein A-I in interstitial fluid after administration of intravenous apolipoprotein A-I/lecithin discs in humans
| Autor: | Waldemar L. Olszewski, Roman Hovorka, C. Justin Cooke, M. Nazeem Nanjee, Irina P. Miller, Norman E. Miller |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty food.ingredient Apolipoprotein B Kinetics QD415-436 Biochemistry Lecithin Endocrinology food Interstitial fluid Internal medicine lymph medicine Humans Infusions Intravenous tissue fluid Serum Albumin Distribution Volume Apolipoprotein A-I biology Chemistry lipoprotein Albumin Extracellular Fluid Cell Biology high density lipoprotein Phosphatidylcholines biology.protein lipids (amino acids peptides and proteins) Lymph interstitium Lipoprotein |
| Zdroj: | Journal of Lipid Research, Vol 47, Iss 5, Pp 975-981 (2006) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.m500358-jlr200 |
| Popis: | Apolipoprotein kinetics are customarily determined by modeling time curves of specific radioactivity or isotopic enrichment in plasma after intravenous infusion of radiolabeled lipoproteins or stable isotope-enriched amino acids. However, this provides no information on the fractional rate of transfer of the apolipoprotein from plasma to interstitial fluid (k(p-if)) or its mean residence time in interstitial fluid (MRT(if)). To determine these parameters for a pharmacologic dose of exogenous apolipoprotein A-I (apoA-I) given intravenously as apoA-I/lecithin discs, we measured apoA-I in plasma and prenodal leg lymph in five healthy men before, during, and after a 4 h infusion at 10 mg/kg/h. ApoA-I concentrations in plasma and lymph were modeled by linear compartmental models (SAAM II version 1.1), using lymph albumin to adjust for the effects of variations in lymph flow rate. k(p-if) averaged 0.75%/h (range, 0.33-1.32), and MRT(if) averaged 29.1 h (14.1-40.0). Neither parameter was correlated with the distribution volume (57-105 ml/kg) or the fractional elimination rate (1.44-2.91%/h) of apoA-I, determined by modeling plasma apoA-I concentration alone. Although used here to study the mass kinetics of apoA-I, if combined with infusion of a tracer, analysis of lymph could also expand the modeling of endogenous apolipoprotein kinetics. |
| Databáze: | OpenAIRE |
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