736 MicroRNA expression patterns in melanomas originating from gynecologic sites
| Autor: | Paolo Fadda, Maribelle Moufawad, Zoe Barricklow, Alejandro A. Gru, Lianbo Yu, Mallory J. DiVincenzo, William E. Carson, Casey Ren, Sarah B. Peters, Lorena P. Suarez-Kelly |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Biology medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens lcsh:RC254-282 Fold change 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Tumor progression 030220 oncology & carcinogenesis Gene expression microRNA Cutaneous melanoma Cancer research medicine Vaginal Melanoma Vulvar melanoma |
| Zdroj: | Journal for ImmunoTherapy of Cancer, Vol 8, Iss Suppl 3 (2020) |
| ISSN: | 2051-1426 |
| Popis: | Background Melanomas originating from gynecologic sites (MOGS) are rare mucosal melanomas originating from the vulva, vagina, and cervix. MOGS are associated with a poor survival rate and limited therapeutic options, as patients often present an advanced disease stage. MiRNAs (miRs) are a class of small, non-coding RNA molecules composed of 21–23 nucleotides that control expression of target genes via post-transcriptional regulation and can exhibit dysregulated expression in cancer. Patterns of miR expression and their effects on disease progression have not yet been explored in the setting of MOGS. We hypothesize a unique miR expression profile exists in MOGS that can mediate disease progression via interaction with target genes. Methods RNA was isolated from formalin fixed, paraffin embedded tissue samples of human vaginal and vulvar melanoma for comparison to normal adjacent vaginal mucosal tissue (NAT) and primary cutaneous melanoma (PCM), respectively. miR expression was then quantified using the NanoString human miRNA assay. Common experimentally validated gene targets of differentially expressed (DE) miRs were identified using miRNet, and pathway analysis was completed to examine potential downstream effects of dysregulated miR expression. Results Comparison of miR expression in vaginal melanoma to NAT revealed 25 DE miRs (fold change > 1.5, p 2, p Conclusions The results of this study support miRNAs as important potential regulators of gene expression in vaginal and vulvar melanomas that can contribute to tumor progression, tumor immunogenicity, and response to current immunotherapies. Ethics Approval This study was approved by the Ohio State University Institutional Review Board, approval #2007C0015. |
| Databáze: | OpenAIRE |
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