SMALL ORGAN4 Is a Ribosome Biogenesis Factor Involved in 5.8S Ribosomal RNA Maturation
| Autor: | Raquel Sarmiento-Mañús, Julio Sáez-Vásquez, Sara Fontcuberta-Cervera, Rosa Micol-Ponce, María Rosa Ponce, Anne de Bures, Adrián Cabezas-Fuster |
|---|---|
| Přispěvatelé: | Universidad Miguel Hernández [Elche] (UMH), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), ANR-17-CE12-0026,RiboStress,Interconnections génétiques, épigénétiques et protéiques au cours de la réorganisation fonctionnelle du nucléole et des réponses aux stress(2017), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
biology Physiology Eukaryotic Large Ribosomal Subunit [SDV]Life Sciences [q-bio] Saccharomyces cerevisiae 5.8S ribosomal RNA Ribosome biogenesis Plant Science Ribosomal RNA biology.organism_classification 01 natural sciences Ribosome Cell biology Arabidopsis Genetics Eukaryotic Small Ribosomal Subunit ComputingMilieux_MISCELLANEOUS 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2020, 184 (4), pp.2022-2039. ⟨10.1104/pp.19.01540⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.19.01540 |
| Popis: | Ribosome biogenesis is crucial for cellular metabolism and has important implications for disease and aging. Human (Homo sapiens) glioma tumor-suppressor candidate region gene2 (GLTSCR2) and yeast (Saccharomyces cerevisiae) Nucleolar protein53 (Nop53) are orthologous proteins with demonstrated roles as ribosome biogenesis factors; knockdown of GLTSCR2 impairs maturation of 18S and 5.8S ribosomal RNAs (rRNAs), and Nop53 is required for maturation of 5.8S and 25S rRNAs. Here, we characterized SMALL ORGAN4 (SMO4), the most likely ortholog of human GLTSCR2 and yeast Nop53 in Arabidopsis (Arabidopsis thaliana). Loss of function of SMO4 results in a mild morphological phenotype; however, we found that smo4 mutants exhibit strong cytological and molecular phenotypes: nucleolar hypertrophy and disorganization, overaccumulation of 5.8S and 18S rRNA precursors, and an imbalanced 40S:60S ribosome subunit ratio. Like yeast Nop53 and human GLTSCR2, Arabidopsis SMO4 participates in 5.8S rRNA maturation. In yeast, Nop53 cooperates with mRNA transport4 (Mtr4) for 5.8S rRNA maturation. In Arabidopsis, we found that SMO4 plays similar roles in the 5.8S rRNA maturation pathway than those described for MTR4. However, SMO4 seems not to participate in the degradation of by-products derived from the 5'-external transcribed spacer (ETS) of 45S pre-rRNA, as MTR4 does. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|