Effect of vaccination on pneumococci isolated from the nasopharynx of healthy children and the middle ear of children with otitis media in Iceland

Autor: Samuel Sigurdsson, Helga Erlendsdóttir, Angela B. Brueggemann, Andries J. van Tonder, Gunnsteinn Haraldsson, Ásgeir Haraldsson, Martha Á. Hjálmarsdóttir, Stephen D. Bentley, Karl G. Kristinsson, Birgir Hrafnkelsson, Sigríður Júlía Quirk
Přispěvatelé: Læknadeild (HÍ), Faculty of Medicine (UI), Lífvísindasetur (HÍ), Biomedical Center (UI), Raunvísindadeild (HÍ), Faculty of Physical Sciences (UI), Heilbrigðisvísindasvið (HÍ), School of Health Sciences (UI), Verkfræði- og náttúruvísindasvið (HÍ), School of Engineering and Natural Sciences (UI), Háskóli Íslands, University of Iceland, Wellcome Trust, GlaxoSmithKline, John Fell Fund, University of Oxford
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Serotype
Eyrnabólga
Epidemiology
Iceland
HAEMOPHILUS-INFLUENZAE
medicine.disease_cause
molecular epidemiology
DISEASE
Pneumococcal Vaccines
EMERGENCE
Streptococcus pneurnoniae
Drug Resistance
Multiple
Bacterial
Nasopharynx
vaccine
STREPTOCOCCUS-PNEUMONIAE SEROTYPES
EPIDEMIOLOGY
Pneumókokkar
Child
education.field_of_study
PENICILLIN-RESISTANT PNEUMOCOCCI
Vaccination
11 Medical And Health Sciences
Pneumococcus
Anti-Bacterial Agents
3. Good health
PCR
Streptococcus pneumoniae
Child
Preschool
Molecular epidemiology
Carrier State
medicine.symptom
Life Sciences & Biomedicine
pneumococcus
CONJUGATE VACCINE
Microbiology (medical)
030106 microbiology
Population
Ear
Middle
UNITED-STATES
Microbial Sensitivity Tests
Serogroup
complex mixtures
Microbiology
Pneumococcal Infections
03 medical and health sciences
stomatognathic system
otorhinolaryngologic diseases
medicine
Humans
Serotyping
education
Otitis media
carriage
Carriage
Science & Technology
business.industry
Faraldsfræði
Infant
Newborn
Infant
otitis media
06 Biological Sciences
Bóluefni
vaccination
Bólusetningar
Multiple drug resistance
Otitis
07 Agricultural And Veterinary Sciences
business
Vaccine
Genome
Bacterial
Multilocus Sequence Typing
Zdroj: Journal of Clinical Microbiology
Popis: Publisher's version (útgefin grein)
Vaccination with pneumococcal conjugate vaccines (PCVs) disrupts the pneumococcal population. Our aim was to determine the impact of the 10-valent PCV on the serotypes, genetic lineages, and antimicrobial susceptibility of pneumococci isolated from children in Iceland. Pneumococci were collected between 2009 and 2017 from the nasopharynges of healthy children attending 15 day care centers and from the middle ears (MEs) of children with acute otitis media from the greater Reykjavik capital area. Isolates were serotyped and tested for antimicrobial susceptibility. Whole-genome sequencing (WGS) was performed on alternate isolates from 2009 to 2014, and serotypes and multilocus sequence types (STs) were extracted from the WGS data. Two study periods were defined: 2009 to 2011 (PreVac) and 2012 to 2017 (PostVac). The overall nasopharyngeal carriage rate was similar between the two periods (67.3% PreVac and 61.5% PostVac, P = 0.090). Vaccine-type (VT) pneumococci decreased and nonvaccine-type (NVT) pneumococci (serotypes 6C, 15A, 15B/C, 21, 22F, 23A, 23B, 35F, and 35B) significantly increased in different age strata post-PCV introduction. The total number of pneumococci recovered from ME samples significantly decreased as did the proportion that were VTs, although NVT pneumococci (6C, 15B/C, 23A, and 23B) increased significantly. Most serotype 6C pneumococci were multidrug resistant (MDR). Serotype 19F was the predominant serotype associated with MEs, and it significantly decreased post-PCV introduction: these isolates were predominantly MDR and of the Taiwan19F-14 PMEN lineage. Overall, the nasopharyngeal carriage rate remained constant and the number of ME-associated pneumococci decreased significantly post-PCV introduction; however, there was a concomitant and statistically significant shift from VTs to NVTs in both collections of pneumococci.
This was an investigator-initiated study funded by GlaxoSmithKline Biologicals SA. Grants were received from the Landspitali University Hospital Research Fund, the Eimskip University Fund, the Wellcome Trust (research fellowship 083511/Z/07/Z and grant 04992/Z/14/Z to A.B.B.), and the John Fell Fund (grant 123/734 to A.B.B.). Work at the Wellcome Sanger Institute was supported by the Wellcome core funding (grant 206194 to S.D.B.)
Databáze: OpenAIRE
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