SPE-HPLC method for the determination and pharmacokinetic studies on paeoniflorin in rat serum after oral administration of traditional Chinese medicinal preparation Guan-Xin-Er-Hao decoction
| Autor: | Hong-Zhu Guo, Yan-Yan Li, Guan Ye, De-An Guo, Yin-Zeng Li |
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| Rok vydání: | 2003 |
| Předmět: |
Bridged-Ring Compounds
Male Analyte Clinical Biochemistry Administration Oral Pharmaceutical Science Decoction Benzoates High-performance liquid chromatography Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Glucosides Pharmacokinetics Drug Discovery Animals Solid phase extraction Medicine Chinese Traditional Chromatography High Pressure Liquid Spectroscopy Detection limit Chromatography Chemistry Paeoniflorin Rats Monoterpenes Quantitative analysis (chemistry) Drugs Chinese Herbal |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 33:521-527 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(03)00294-2 |
| Popis: | A new HPLC method for the determination of paeoniflorin in rat serum with solid-phase extraction (SPE) for preconcentration is introduced. Paeoniflorin and an internal standard (pentoxifylline) were extracted from serum by means of SPE using cartridges with octadecyl chemically bound phase. The HPLC separation was then performed on a reversed-phase C(18) column using acetonitrile-water (18:82, v/v) as eluting solvent system, and UV detection at 230 nm to measure the analyte with a limit of quantitation about 10 ng ml(-1). The calibration curve for paeoniflorin was linear (r=0.9938) in the concentration range of 10-1200 ng ml(-1), both intra- and inter-day precision of the paeoniflorin were determined and their coefficience of variation did not exceed 10%. The validated method has been successfully applied for pharmacokinetic studies of paeoniflorin from rat serum after oral administration of Guan-Xin-Er-Hao decoction. |
| Databáze: | OpenAIRE |
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