Lack of claudin-7 is a strong predictor of regional recurrence in oral and oropharyngeal squamous cell carcinoma

Autor: Ben N G Giepmans, Ulrike Schnell, Jan L. N. Roodenburg, Lieuwe J. Melchers, Mirjam F. Mastik, van der Bernard Laan, de Truuske Bock, Ed Schuuring, van Boukje Dijk, J.E. van der Wal, L. Bruine de Bruin, Lorian Slagter-Menkema
Přispěvatelé: Faculteit Medische Wetenschappen/UMCG, Damage and Repair in Cancer Development and Cancer Treatment (DARE), Life Course Epidemiology (LCE), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Man, Biomaterials and Microbes (MBM), Targeted Gynaecologic Oncology (TARGON), Center for Liver, Digestive and Metabolic Diseases (CLDM)
Rok vydání: 2013
Předmět:
Male
Oncology
DOWN-REGULATION
Cancer Research
Head and neck cancer
Claudin-7
TUMOR PROGRESSION
Lymph node
Aged
80 and over
Tissue microarray
Oral cancer
CATENIN EXPRESSION
Middle Aged
Cadherins
Epithelial Cell Adhesion Molecule
Prognosis
EPCAM EXPRESSION
Oropharyngeal Neoplasms
medicine.anatomical_structure
Carcinoma
Squamous Cell
Immunohistochemistry
Female
Mouth Neoplasms
Oral Surgery
Adult
medicine.medical_specialty
Regional recurrence
Biology
ELECTIVE NECK DISSECTION
Nodal metastasis
ADHESION MOLECULES
E-CADHERIN EXPRESSION
Antigens
Neoplasm
Internal medicine
Biomarkers
Tumor
medicine
Humans
Claudin
Aged
Cadherin
HUMAN-PAPILLOMAVIRUS
LYMPH-NODE METASTASIS
Cell adhesion
E-cadherin
medicine.disease
Tumor progression
EpCAM
Claudins
EP-CAM EXPRESSION
Lymph Nodes
Neoplasm Recurrence
Local
NODAL
Cell Adhesion Molecules
Zdroj: Oral Oncology, 49(10), 998-1005. ELSEVIER SCIENCE BV
ISSN: 1368-8375
Popis: Objectives: Adequate treatment of oral and oropharyngeal squamous cell carcinoma (OSCC) is dependent on correctly predicting the presence of lymph node metastases. Current methods to diagnose nodal metastases partly result in overtreatment with associated morbidity and undertreatment with decreased disease-free survival. E-cadherin has been studied extensively as potential marker for lymph node metastases. EpCAM and claudin-7 have a functional relationship with E-cadherin, forming a complex that promotes tumourigenicity in vitro. We hypothesize that the co-expression patterns of these related molecules is a better prognostic marker for nodal status and regional recurrences.Materials and methods: We constructed separate tissue microarrays of tumour centre and tumour invasive front of 227 OSCC with complete clinicopathological and follow-up data, including HPV status, and performed immunohistochemistry for these molecules.Results: Lack of E-cadherin and presence of cytoplasmic EpCAM expression in the tumour front were predictive for nodal metastasis, but no co-expression pattern was found clinically relevant. Lack of claudin-7 in the tumour centre was highly and independently predictive for shorter regional disease-free survival (HR = 0.19; 95% CI: 0.06-0.62) and disease-specific survival (HR = 0.43; 95% CI: 0.21-0.87). High-risk HPV was not associated with any marker.Conclusions: The expression of E-cadherin and EpCAM, depending on the specific tumour sublocalization, is predictive for nodal status. However, co-expression did not improve the prediction of nodal status, indicating that the proposed in vitro complex is not functional in clinical samples. Additionally, lack of claudin-7 expression in the tumour centre may be used to identify patients with increased risk for regional recurrence. (C) 2013 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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