Size- and time-dependent growth properties of human induced pluripotent stem cells in the culture of single aggregate
| Autor: | Masanobu Horie, Masahiro Kino-oka, Eiji Nagamori, Suman Chandra Nath |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors Induced Pluripotent Stem Cells Cell Culture Techniques Cell- and Tissue-Based Therapy Cell Count Bioengineering Nanotechnology Applied Microbiology and Biotechnology Collagen Type I Cell therapy Extracellular matrix 03 medical and health sciences Humans Proliferation Marker Secretion Growth rate Human Induced Pluripotent Stem Cells Autocrine signalling Cell Aggregation Cell Proliferation Cell Size Aggregate (composite) Chemistry Culture Media 030104 developmental biology Biophysics Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 124:469-475 |
| ISSN: | 1389-1723 |
| Popis: | Aggregate culture of human induced pluripotent stem cells (hiPSCs) is a promising method to obtain high number of cells for cell therapy applications. This study quantitatively evaluated the effects of initial cell number and culture time on the growth of hiPSCs in the culture of single aggregate. Small size aggregates ((1.1 ± 0.4) × 101–(2.8 ± 0.5) × 101 cells/aggregate) showed a lower growth rate in comparison to medium size aggregates ((8.8 ± 0.8) × 101–(6.8 ± 1.1) × 102 cells/aggregate) during early-stage of culture (24–72 h). However, when small size aggregates were cultured in conditioned medium, their growth rate increased significantly. On the other hand, large size aggregates ((1.1 ± 0.2) × 103–(3.5 ± 1.1) × 103 cells/aggregate) showed a lower growth rate and lower expression level of proliferation marker (ki-67) in the center region of aggregate in comparison to medium size aggregate during early-stage of culture. Medium size aggregates showed the highest growth rate during early-stage of culture. Furthermore, hiPSCs proliferation was dependent on culture time because the growth rate decreased significantly during late-stage of culture (72–120 h) at which point collagen type I accumulated on the periphery of aggregate, suggesting blockage of diffusive transport of nutrients, oxygen and metabolites into and out of the aggregates. Consideration of initial cell number and culture time are important to maintain balance between autocrine factors secretion and extracellular matrix accumulation on the aggregate periphery to achieve optimal growth of hiPSCs in the culture of single aggregate. |
| Databáze: | OpenAIRE |
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