Morphological and chemical analysis of magic mushrooms in Japan
Autor: | Tatsuyuki Kanamori, Ritsuko Sugita, Yoshihito Ohmae, Kenji Tsujikawa, Tohru Kishi, Hiroyuki Inoue, Yuko T. Iwata |
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Rok vydání: | 2003 |
Předmět: |
Psilocybe cubensis
Analytical chemistry High-performance liquid chromatography Pathology and Forensic Medicine Alkaloids Japan medicine Hymenium Chromatography High Pressure Liquid Mushroom Chromatography Psilocybe biology Plant Stems Chemistry Methanol Forensic Medicine Spores Fungal biology.organism_classification Basidium Psilocybin Copelandia Psilocin Hallucinogens Microscopy Electron Scanning Solvents Agaricales Law medicine.drug |
Zdroj: | Forensic science international. 138(1-3) |
ISSN: | 0379-0738 |
Popis: | Morphological and toxicological analyses were performed on hallucinogenic mushrooms that are currently circulated in Japan. Scanning electron microscope (SEM) indicated a three-dimensional microstructures in the mushrooms. The complementary use of SEM with an optical microscope was effective for observing characteristic tissues, such as basidiomycetes, spores, cystidia and basidia. Hallucinogenic alkaloids were extracted with methanol and determined by high performance liquid chromatography (HPLC) with a UV detector set at 220nm. The psilocin/psilocybin contents in Psilocybe cubensis were in the range of 0.14–0.42%/0.37–1.30% in the whole mushroom (0.17–0.78%/0.44–1.35% in the cap and 0.09–0.30%/0.05–1.27% in the stem), respectively. The hallucinogenic alkaloids in Copelandia were 0.43–0.76%/0.08–0.22% in the whole mushroom (0.64–0.74%/0.02–0.22% in the cap and 0.31–0.78%/0.01–0.39% in the stem). It thus appears that P. cubensis is psilocybin-rich, whereas Copelandia is psilocin-rich. |
Databáze: | OpenAIRE |
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