Comparison of DNA Damage and Oxidative Stress in Patients Anesthetized With Desflurane Associated or Not With Nitrous Oxide
| Autor: | Mariana Gobbo Braz, Chung-Yen Chen, Nayara M. Arruda, Kátina M. Souza, Lídia Raquel de Carvalho, José Reinaldo Cerqueira Braz, Aline G. Aun, Flávia R. Nogueira, Leandro Gobbo Braz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Time Factors Adolescent DNA damage Nitrous Oxide medicine.disease_cause law.invention Protein Carbonylation Lipid peroxidation Young Adult 03 medical and health sciences chemistry.chemical_compound Desflurane 0302 clinical medicine Randomized controlled trial 030202 anesthesiology law Administration Inhalation Humans Medicine Prospective Studies Prospective cohort study Inhalation business.industry Nitrous oxide Middle Aged equipment and supplies Anesthetics Combined Oxidative Stress 030104 developmental biology Anesthesiology and Pain Medicine chemistry Anesthesia Anesthetics Inhalation Female Lipid Peroxidation Anesthesia Inhalation business Biomarkers Brazil Oxidative stress DNA Damage medicine.drug |
| Zdroj: | Anesthesia & Analgesia. 126:1198-1205 |
| ISSN: | 0003-2999 |
| DOI: | 10.1213/ane.0000000000002729 |
| Popis: | Little is known about the effects of desflurane associated or not with nitrous oxide (N2O) on oxidative stress and patient genetic material. The aim of this study was to compare the effects of anesthesia maintained with desflurane associated or not with N2O on DNA damage (as a primary outcome) and oxidative stress (as a secondary outcome) in patients who underwent an elective minimally invasive surgery.This prospective randomized clinical trial analyzed 40 patients of both sexes with an American Society of Anesthesiologists physical status I who were 18-50 years of age and scheduled for septoplasty. The patients were randomly allocated into 2 groups according to anesthesia maintenance as follows: desflurane (n = 20) or desflurane/N2O (n = 20). Blood samples were collected before anesthesia (T1 = baseline), 1.5 hours after anesthesia induction (T2), and on the morning of the postoperative first day (T3). Basal and oxidative DNA damage (determined using formamidopyrimidine DNA glycosylase to detect oxidized purines and endonuclease III to detect oxidized pyrimidines) were evaluated using the comet assay. Oxidative stress markers were evaluated based on lipid peroxidation (by assessing 4-hydroxynonenal and 8-iso-prostaglandin F2α [8-isoprostane] using enzyme linked immunosorbent immunoassay), protein carbonyls (assessed by enzyme linked immunosorbent immunoassay), and antioxidant defense (ferric-reducing antioxidant power by spectrophotometry). The effect size was expressed as the mean differences between groups and the corresponding 95% confidence interval (CI).There was no significant mean difference between groups in relation to DNA damage (-1.7 [95% CI, -7.0 to 3.5]), oxidized DNA pyrimidines (-1.8 [95% CI, -12.5 to 8.9]) and purines (-1.9 [95% CI, -13.9 to 10.1]), 4-hydroxynonenal (-0.2 [95% CI, -2.8 to 2.4]), 8-isoprostane (549 [95% CI, -2378 to 3476]), protein carbonyls (0.2 [95% CI, -2.1 to 2.3]), or ferric-reducing antioxidant power (24 [95% CI, -52.0 to 117.2]).The coadministration of 60% N2O with desflurane did not seem to impair the effects on DNA or the redox status compared with desflurane anesthesia, suggesting that both studied anesthetic techniques can be suitable options for healthy individuals who undergo minimally invasive surgery lasting at least 1.5 hours. However, due to the low power of the study, more research is necessary to confirm our findings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|