In vitro plant regeneration of Aster scaber via somatic embryogenesis
| Autor: | Reniel S. Pamplona, Dong-Sun Lee, Kyung Hwan Boo, Key-Zung Riu, Doseung Lee, Dang Viet Cao |
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| Rok vydání: | 2015 |
| Předmět: |
Plant Somatic Embryogenesis Techniques
Somatic embryogenesis Aster Plant Biology In Vitro Techniques Applied Microbiology and Biotechnology Biochemistry Plant Roots Naphthaleneacetic Acids Analytical Chemistry chemistry.chemical_compound Tissue culture Soil Plant Growth Regulators Auxin Botany Benzyl Compounds Regeneration Molecular Biology chemistry.chemical_classification Indoleacetic Acids fungi Organic Chemistry food and beverages General Medicine Kinetin Micropropagation chemistry Purines Callus Shoot Cytokinin Plant Shoots Biotechnology Explant culture |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 79(5) |
| ISSN: | 1347-6947 |
| Popis: | We established an in vitro plant regeneration system via somatic embryogenesis of Aster scaber, an important source of various biologically active phytochemicals. We examined the callus induction and embryogenic capacities of three explants, including leaves, petioles, and roots, on 25 different media containing different combinations of α-naphthalene acetic acid (NAA) and 6-benzyladenine (BA). The optimum concentrations of NAA and BA for the production of embryogenic calli were 5.0 μM and 0.05 μM, respectively. Media containing higher concentrations of auxin and cytokinin (such as 25 μM NAA and 25 μM BA) were suitable for shoot regeneration, especially for leaf-derived calli, which are the most readily available calli and are highly competent. For root induction from regenerated shoots, supplemental auxin and/or cytokinin did not improve rooting, but instead caused unwanted callus induction or retarded growth of regenerated plants. Therefore, plant growth regulator-free medium was preferable for root induction. Normal plants were successfully obtained from calli under the optimized conditions described above. This is the first report of the complete process of in vitro plant regeneration of A. scaber via somatic embryogenesis. |
| Databáze: | OpenAIRE |
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