Interactions in vitro and in vivo between Human and Porcine Cationic Pancreatic Elastase and Plasma Protease Inhibitors
| Autor: | Hans-Olof Håkansson, Kjell Ohlsson |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Protease Pancreatic Elastase Swine Chemistry medicine.medical_treatment Elastase Cationic polymerization Preferential binding Biochemistry In vitro Kinetics In vivo medicine Animals Humans Female Protease Inhibitors alpha-Macroglobulins Immunoelectrophoresis Two-Dimensional Pancreas Blood stream Pancreatic elastase Protein Binding |
| Zdroj: | Biological Chemistry Hoppe-Seyler. 369:309-316 |
| ISSN: | 0177-3593 |
| Popis: | Trace amounts of porcine pancreatic elastase mixed with porcine serum, or injected intravenously into the pig, were found to be bound mainly to alpha 1- and alpha 2-macroglobulin (90%). Alpha 1-macroglobulin approached saturation with elastase before significant binding to alpha 2-macroglobulin was demonstrable. Human pancreatic cationic elastase showed in human serum preferential binding to alpha 2-macroglobulin, but the elastase was also bound by alpha 1-protease inhibitor and by alpha 1-antichymotrypsin. The porcine elastase-alpha 1 alpha 2-macroglobulin complexes injected intravenously or formed in vivo in the pig were rapidly eliminated from the blood stream following a first order reaction with t 1/2 = 8 min. Porcine alpha 1-protease-inhibitor-bound elastase disappeared considerably more slowly. |
| Databáze: | OpenAIRE |
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