Biological Activity of Cannabis sativa L. Extracts Critically Depends on Solvent Polarity and Decarboxylation
Autor: | Carlos Ferreiro Vera, Carolina Sánchez-Carnerero, Xavier Nadal Roura, Guillermo Moreno-Sanz, Verónica Sánchez de Medina Baena |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
cannabis
THC Decarboxylation medicine.medical_treatment Filtration and Separation Analytical Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound cannabinoids 0302 clinical medicine medicine decarboxylation Ethanol Chemotype biology Biological activity biology.organism_classification In vitro lcsh:QC1-999 botanical extracts Solvent chemistry Biochemistry lcsh:QD1-999 030220 oncology & carcinogenesis CBD Cannabinoid Cannabis 030217 neurology & neurosurgery lcsh:Physics |
Zdroj: | Separations, Vol 7, Iss 56, p 56 (2020) Separations Volume 7 Issue 4 |
ISSN: | 2297-8739 |
Popis: | Minor cannabinoid and non-cannabinoid molecules have been proposed to significantly contribute to the pharmacological profile of cannabis extracts. Phytoplant Research has developed highly productive cannabis cultivars with defined chemotypes, as well as proprietary methods for the extraction and purification of cannabinoids. Here, we investigate the effect of solvent selection and decarboxylation on the composition and pharmacological activity of cannabis extracts. A library of forty cannabis extracts was generated from ten different cannabis cultivars registered by Phytoplant Research at the EU Community Plant Variety Office. Plant material was extracted using two different solvents, ethanol and hexane, and crude extracts were subsequently decarboxylated or not. Cannabinoid content in the resulting extracts was quantified, and biological activity was screened in vitro at three molecular targets involved in hypoxia and inflammation (NF-&kappa B, HIF-1&alpha and STAT3). Changes in transcriptional activation were strongly associated to solvent selection and decarboxylation. Two decarboxylated extracts prepared with hexane were the most potent at inhibiting NF-&kappa B transcription, while HIF-1&alpha activation was preferentially inhibited by ethanolic extracts, and decarboxylated extracts were generally more potent at inhibiting STAT3 induction. Our results indicate that solvent selection and proper decarboxylation represent key aspects of the standardized production of cannabis extracts with reproducible pharmacological activity. |
Databáze: | OpenAIRE |
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