A simple in vitro biomimetic perfusion system for mechanotransduction study
| Autor: | Sarah Cartmell, Ruikang Xue, Christodoulos Stefanadis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Texture measurement Materials science 211 Scaffold / Tissue engineering/Drug delivery 02 engineering and technology 010402 general chemistry 01 natural sciences flow-induced shear stress General Materials Science Mechanotransduction Cytoskeleton Materials of engineering and construction. Mechanics of materials Actin mesenchymal stem cell Bio-Inspired and Biomedical Materials mechanotransduction Mesenchymal stem cell in vitro 021001 nanoscience & nanotechnology In vitro biomimetic perfusion system 0104 chemical sciences TA401-492 0210 nano-technology Perfusion TP248.13-248.65 Biomedical engineering Research Article Biotechnology |
| Zdroj: | Science and Technology of Advanced Materials, Vol 21, Iss 1, Pp 635-640 (2020) Science and Technology of Advanced Materials article-version (VoR) Version of Record Xue, R & Cartmell, S 2020, ' A simple in vitro biomimetic perfusion system for mechanotransduction study ', Science and Technology of Advanced Materials, vol. 21, no. 1, pp. 635-640 . https://doi.org/10.1080/14686996.2020.1808432 |
| ISSN: | 1878-5514 1468-6996 |
| Popis: | In mechanotransduction studies, flow-induced shear stress (FSS) is often applied to two-dimensional (2D) cultured cells with a parallel-plate flow chamber (PPFC) due to its simple FSS estimation. However, cells behave differently under FSS inside a 3D scaffold (e.g. 10 mPa FSS was shown to induce osteogenesis of human mesenchymal stem cells (hMSC) in 3D but over 900 mPa was needed for 2D culture). Here, a simple in vitro biomimetic perfusion system using borosilicate glass capillary tubes has been developed to study the cellular behaviour under low-level FSS that mimics 3D culture. It has been shown that, compared to cells in the PPFC, hMSC in the capillary tubes had upregulated Runx-2 expression and osteogenic cytoskeleton actin network under 10 mPa FSS for 24 h. Also, an image analysis method based on Haralick texture measurement has been used to identify osteogenic actin network. The biomimetic perfusion system can be a valuable tool to study mechanotransduction in 3D for more clinical relevant tissue-engineering applications. Graphical abstract |
| Databáze: | OpenAIRE |
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