Cooperation of sonic hedgehog enhancers in midline expression
| Autor: | Nadine Fischer, Uwe Strähle, Ferenc Müller, Yavor Hadzhiev, Sepand Rastegar, Saradavey Rathnam, Raymond Ertzer |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Rok vydání: | 2006 |
| Předmět: |
Embryo
Nonmammalian Time Factors Gene Expression MESH: Prosencephalon Genes Reporter MESH: Gene Expression Regulation Developmental MESH: Animals Sonic hedgehog Zebrafish Genetics Regulation of gene expression 0303 health sciences MESH: Enhancer Elements (Genetics) 030302 biochemistry & molecular biology MESH: Notochord Gene Expression Regulation Developmental medicine.anatomical_structure Enhancer Elements Genetic Somites Regulatory sequence embryonic structures animal structures MESH: Gene Expression Zona limitans intrathalamica Hypothalamus Notochord Biology Transgene MESH: Somites 03 medical and health sciences Prosencephalon medicine Animals Hedgehog Proteins Enhancer MESH: Zebrafish Molecular Biology 030304 developmental biology Floor plate MESH: Time Factors MESH: Genes Reporter MESH: Embryo Nonmammalian [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology MESH: Hedgehog Proteins Cell Biology biology.organism_classification MESH: Hypothalamus Embryonic shield biology.protein Developmental Biology |
| Zdroj: | Developmental Biology Developmental Biology, Elsevier, 2007, 301 (2), pp.578-89. ⟨10.1016/j.ydbio.2006.11.004⟩ |
| ISSN: | 0012-1606 1095-564X |
| DOI: | 10.1016/j.ydbio.2006.11.004⟩ |
| Popis: | International audience; In zebrafish, as in other vertebrates, the secreted signalling molecule Sonic hedgehog (Shh) is expressed in organiser regions such as the embryonic midline and the zona limitans intrathalamica (zli). To investigate the regulatory mechanisms underlying the pattern of shh expression, we carried out a systematic analysis of the intronic regulatory sequences of zebrafish shh using stable transgenesis. Deletion analysis identified the modules responsible for expression in the embryonic shield, the hypothalamus and the zli and confirmed the activities of previously identified notochord and floor plate enhancers. We detected a strong synergism between regulatory regions. The degree of synergy varied over time in the hypothalamus suggesting different mechanisms for initiation and maintenance of expression. Our data show that the pattern of shh expression in the embryonic central nervous system involves an intricate crosstalk of at least 4 different regulatory regions. When compared to the enhancer activities of the mouse Shh gene, we observed a remarkable divergence of function of structurally conserved enhancer sequences. The activating region ar-C (61% identical to SFPE2 in mouse Shh), for example, mediates floor plate expression in the mouse embryo while it directs expression in the forebrain and the notochord and only weakly in the floor plate in the zebrafish embryo. This raises doubts on the predictive power of phylogenetic footprinting and indicates a stunning divergence of function of structurally conserved regulatory modules during vertebrate evolution. |
| Databáze: | OpenAIRE |
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