Combined GTG-banding and nonradioactive in situ hybridization improves characterization of complex karyotypes
| Autor: | P. Mollevanger, A.K. Raap, C.J. Cornelisse, Geoffrey C. Beverstock, Vincent T.H.B.M. Smit, P.I. Schrier, J.W. Wessels |
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| Rok vydání: | 1990 |
| Předmět: |
In situ
Adult Male medicine.medical_specialty Centromere In situ hybridization Biology Genetics medicine Tumor Cells Cultured Humans Trypsin Gtg banding Molecular Biology Genetics (clinical) Cells Cultured Chromosome Aberrations Ovarian Neoplasms Cytogenetics Nucleic Acid Hybridization Karyotype Molecular biology Molecular hybridization Chromosome Banding Karyotyping Female Molecular probe DNA Probes |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0301-0171 |
| Popis: | Nonradioactive in situ hybridization (ISH) using bio-tinylated centromere probes for chromosomes 1, 6, 7, 10, 16, 17, 18, and the X, respectively, was combined with GTG-banding to study cytogenetic changes in two different ovarian cancer cell lines. ISH was performed after GTG-banding on the same metaphase. The use of a low trypsin concentration (0.01%) in the banding procedure was essential for subsequent ISH. This combined approach allows the detection of subtle chromosomal rearrangements and appears to aid the identification of marker chromosomes. |
| Databáze: | OpenAIRE |
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