AMPAR/TARP stoichiometry differentially modulates channel properties
| Autor: | Nuria Sánchez-Fernández, David Soto, Federico Miguez-Cabello, Xavier Gasull Casanova, Retic Oftared, Esther Gratacòs-Batlle, Natalia Yefimenko, Sgr |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type Patch-Clamp Techniques Mouse QH301-705.5 Science Primary Cell Culture Endogeny AMPA receptor AMPAR TARP General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Cerebellum Animals Humans Biology (General) Receptor Electrofisiologia Neural transmission General Immunology and Microbiology Chemistry General Neuroscience musculoskeletal neural and ocular physiology General Medicine out-side out stoichiometry Electrophysiology Mice Inbred C57BL 030104 developmental biology HEK293 Cells nervous system Receptors Glutamate cerebellar granule cells Biophysics Medicine Neurotransmissió channel conductance Calcium Channels 030217 neurology & neurosurgery Stoichiometry Research Article Neuroscience Human |
| Zdroj: | eLife Dipòsit Digital de la UB Universidad de Barcelona eLife, Vol 9 (2020) |
| ISSN: | 2050-084X |
| Popis: | SummaryAMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain AMPAR properties. Here we have found that AMPARs show a clear stoichiometry dependent modulation although AMPARs still need to be fully saturated with TARPs to show some typical TARP-induced characteristics (i.e. an increase in channel conductance). We also have uncovered important differences in the stoichiometric modulation between calcium-permeable and calcium-impermeable AMPARs. Moreover, in heteromeric AMPARs, TARP positioning in the complex is important to exert certain TARP-dependent features. Finally, by comparing data from recombinant receptors with endogenous AMPAR currents from cerebellar granule cells, we have determined a likely functional stoichiometry of 2 TARPs associated with GluA2 subunits in the somatic AMPARs found in this cell type. |
| Databáze: | OpenAIRE |
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