DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA)
| Autor: | Nicoline F. Tanih, Lucy M. Ndip, Roland N. Ndip |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Sequence analysis Virulence Factors Population Bacterial Toxins Virulence Catalysis Article Microbiology diversity Inorganic Chemistry lcsh:Chemistry South Africa Bacterial Proteins Genetic variation vacuolating cytotoxin gene (vacA) Humans Physical and Theoretical Chemistry education Molecular Biology Gene lcsh:QH301-705.5 Spectroscopy Genetics education.field_of_study Genetic diversity biology Helicobacter pylori Organic Chemistry Genetic Variation General Medicine Sequence Analysis DNA biology.organism_classification Amplicon Size Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 |
| Zdroj: | International Journal of Molecular Sciences, Vol 12, Iss 11, Pp 7459-7468 (2011) International Journal of Molecular Sciences Volume 12 Issue 11 Pages 7459-7468 |
| ISSN: | 1422-0067 |
| Popis: | Sequence diversity and population structures can vary widely among pathogenic bacteria species. In some species, all isolates are highly similar, whereas in others most of the isolates are distinguished easily. H. pylori is known for its wide genetic diversity amongst the various strains most especially in the genes involved in virulence. The aim of this study was to evaluate by PCR and sequence analysis, the genetic profile of H. pylori vacA gene (s1, s2, m1 and m2). We sequenced small DNA segments from 13 vacAs1, 10 vacAm2, 6 vacAm1 and 6 vacAs2 strains which were amplified with amplicon size of 259/286 bp, 290 bp and 352 bp for vacAs1/s2, m1 and m2 respectively. Based on similarities among our strains accession numbers were provided for seven vacAs1 (HQ709109–HQ709115), six vacAs2 (JN848463–JN848468), six vacAm1 (JN848469–JN848474) and six vacAm2 (HQ650801–HQ650806) strains. Amongst the strains studied, 98.07%, 98.58%, 97.38% and 95.41% of vacAs1, vacAs2, vacAm1 and vacAm2 of the strains were conserved respectively. Findings of this study underscores the importance of understanding the virulence composition and diversity of H. pylori in South Africa for enhanced clinico-epidemiological monitoring and pathophysiology of disease. |
| Databáze: | OpenAIRE |
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