Transmission-blocking compound candidates against Plasmodium vivax using P. berghei as an initial screening
| Autor: | Wuelton Marcelo Monteiro, Alexandre Oliveira Trindade, Macejane Ferreira Souza, Stefanie C. P. Lopes, Fabio T. M. Costa, Rogerio Amino, Claudia María Ríos-Velásquez, Marcus V. G. Lacerda, Francy's Sayara Andrade, Camila Fabbri |
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| Přispěvatelé: | Fundação de Medicina Tropical Dr Heitor Vieira Dourado [Manaus, Brazil], Universidade do Estado do Amazonas (UEA), Centro Universitário Fametro [Manaus, Brazil], Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Universidade Estadual de Campinas (UNICAMP), Infection et Immunité paludéennes - Malaria Infection and Immunity, Institut Pasteur [Paris], Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Universidade Estadual de Campinas = University of Campinas (UNICAMP), Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Plasmodium berghei [SDV]Life Sciences [q-bio] 030231 tropical medicine Plasmodium vivax RC955-962 Mosquito Vectors Microbiology Flow cytometry 03 medical and health sciences 0302 clinical medicine Arctic medicine. Tropical medicine parasitic diseases medicine Gametocyte Malaria Vivax Animals MESH: Plasmodium berghei MESH: Animals biology medicine.diagnostic_test membrane feeding assay and gametocytes MESH: Malaria Vivax biology.organism_classification medicine.disease Transmission blocking Virology QR1-502 3. Good health MESH: Plasmodium vivax [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Vector (epidemiology) Original Article MESH: Mosquito Vectors Malaria Box Ex vivo Malaria |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 116 (2021) Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Instituto Oswaldo Cruz, Ministério da Saúde, 2021, 116, pp.e200513. ⟨10.1590/0074-02760200513⟩ Memórias do Instituto Oswaldo Cruz, 2021, 116, pp.e200513. ⟨10.1590/0074-02760200513⟩ Memórias do Instituto Oswaldo Cruz, Volume: 116, Article number: e200513, Published: 08 FEB 2021 |
| ISSN: | 1678-8060 0074-0276 |
| DOI: | 10.1590/0074-02760200513⟩ |
| Popis: | International audience; Background: Different strategies for improvement of malaria control and elimination are based on the blockage of malaria parasite transmission to the mosquito vector. These strategies include the drugs that target the plasmodial sexual stages in humans and the early developmental stages inside mosquitoes.Objectives: Here we tested Malaria Box compounds in order to evaluate their activity against male and female gametocytes in Plasmodium berghei, mosquito infection in P. vivax and ookinete formation in both species.Methods/findings: The membrane feeding assay and the development of ookinetes by a 24 h ex vivo culture and the ookinete yield per 1000 erythrocytes were used to test transmission-blocking potential of the Malaria Box compounds in P. vivax. For P. berghei we used flow cytometry to evaluate male and female gametocyte time course and fluorescence microscopy to check the ookinete development. The two species used in this study showed similar results concerning the compounds' activity against gametocytes and ookinetes, which were different from those in P. falciparum. In addition, from the eight Malaria Box compounds tested in both species, compounds MMV665830, MMV665878 and MMV665941 were selected as a hit compounds due the high inhibition observed.Conclusion: Our results showed that P. berghei is suitable as an initial screening system to test compounds against P. vivax. |
| Databáze: | OpenAIRE |
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