LC-MS/MS quantification of sulforaphane and indole-3-carbinol metabolites in human plasma and urine after dietary intake of selenium-fortified broccoli
| Autor: | Veronika Somoza, Stefanie Winkler, Marc Pignitter, Johanna Hauder, Corinna E. Rüfer, Achim Bub |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Indoles Glucosinolates chemistry.chemical_element Urine Brassica law.invention Glucobrassicin chemistry.chemical_compound Selenium law Isothiocyanates Tandem Mass Spectrometry Indole-3-carbinol Humans Food science Fertilizers Chromatography High Pressure Liquid Aged Glucoraphanin Aged 80 and over Dietary intake food and beverages General Chemistry Middle Aged Diet chemistry Biochemistry Sulfoxides Female General Agricultural and Biological Sciences Atomic absorption spectroscopy Thiocyanates Sulforaphane |
| Zdroj: | Journal of agricultural and food chemistry. 59(15) |
| ISSN: | 1520-5118 |
| Popis: | This study aimed at developing a sensitive LC-MS/MS method for the quantification of sulforaphane (SFN) and indole-3-carbinol metabolites in plasma and urine after dietary intake of regular and selenium-fertilized broccoli using stable isotope dilution analysis. In a three-armed, placebo-controlled, randomized human intervention study with 76 healthy volunteers, 200 g of regular (485 μg of total glucosinolates and0.01 μg of selenium per gram fresh weight) or selenium-fertilized broccoli (589 μg of total glucosinolates and 0.25 μg of selenium per gram fresh weight) was administered daily for 4 weeks. Glucoraphanin and glucobrassicin metabolites quantified in plasma and urine were SFN-glutathione, SFN-cysteine, SFN-cysteinylglycine, SFN-acetylcysteine, and indole-3-carboxaldehyde, indole-3-carboxylic acid, and ascorbigen, respectively. Dietary intake of selenium-fertilized broccoli increased serum selenium concentration analyzed by means of atomic absorption spectroscopy by up to 25% (p0.001), but affected neither glucosinolate concentrations in broccoli nor their metabolite concentrations in plasma and urine compared to regular broccoli. |
| Databáze: | OpenAIRE |
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