Application of new fluorescent thiol reagent to diagnosis of homocystinuria
| Autor: | J. L. Maddocks, J. MacLachlan |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Homocysteine Thiocyanate Phenylmercuric Acetate Homocystinuria General Medicine Urine medicine.disease Fluorescence Acetylcysteine chemistry.chemical_compound Biochemistry chemistry Reagent Thiol medicine Humans Chromatography Thin Layer Sulfhydryl Compounds Child Cysteine medicine.drug |
| Zdroj: | Lancet (London, England). 338(8774) |
| ISSN: | 0140-6736 |
| Popis: | A new fluorescent thiol reagent, dansyl-aminophenylmercuric acetate (DAPMA), was applied to the diagnosis of homocystinuria, a disorder which can be associated with vascular disease at an early age. DAPMA was added to urine containing metabisulphite and the resulting fluorescent derivatives were extracted on a cyclohexyl silica column and separated by thin-layer chromatography. 102 coded samples were tested. The derivative of homocysteine was easily identified in samples from 4 children with homocystinuria but was absent from all samples from normal subjects and patients with unrelated disorders. Other thiols (cysteine, acetylcysteine, mercaptolactate, thiosulphate, and thiocyanate) were also identified in urine from healthy fasting subjects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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