Effect of Mycoplasma pulmonis infection on protein and glycosaminoglycan synthesis of cultured connective tissue cells
| Autor: | Timo Kouri, Hilkka Turakainen, Markku Jalkanen, Ronald H. Leach, Risto Penttinen |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Proline
Connective tissue Cell Count Biology Glycosaminoglycan chemistry.chemical_compound Methionine Mycoplasma Hyaluronic acid medicine Protein biosynthesis Animals Humans Hyaluronic Acid Cells Cultured Connective Tissue Cells Glycosaminoglycans Cell Membrane Granulation tissue Cell Biology Rats Procollagen peptidase medicine.anatomical_structure Biochemistry chemistry Connective Tissue Cell culture Protein Biosynthesis Mycoplasma pulmonis Collagen |
| Zdroj: | Experimental Cell Research. 148:1-10 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(83)90182-9 |
| Popis: | Human synovial cells, fetal skin fibroblasts and rat granulation tissue fibroblasts were experimentally infected with Mycoplasma pulmonis, a species identified as a contamination of cell cultures, and studied for collagen, total protein and glycosaminoglycan synthesis. Hyaluronic acid and sulfated glycosaminoglycan synthesis were stimulated in cultures where the infection reduced cell density, while they were retarded in cultures which had proliferated into higher density than the controls. An extra polypeptide with molecular weight of 20 kD was seen in [35S]methionine-labelled cells. Media of rat granulation tissue cells showed a shift of a 39-42 kD polypeptide to 33-36 kD position in [35S]methionine and [3H]proline labellings. Other minor changes were also noticed. Collagen synthesis or procollagen conversion to collagen were, however, not altered. |
| Databáze: | OpenAIRE |
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