CL-L1 and CL-K1 Exhibit Widespread Tissue Distribution With High and Co-Localized Expression in Secretory Epithelia and Mucosa
Autor: | Søren Hansen, Karen B Bjerrum, Karsten Skjoedt, Anna Lahn Sørensen, Maiken Lumby Henriksen, Josephine B Aagaard, Ole Haagen Nielsen, Jonas Heilskov Graversen, Eva Kildall Hejbøl, Henrik Daa Schrøder |
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Rok vydání: | 2018 |
Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine Glycoconjugate Immunology Collectin Kidney Epithelium collectin 03 medical and health sciences Exocrine Glands 0302 clinical medicine mucosal immunology Endocrine Glands Humans Immunology and Allergy 3MC syndrome Complement Activation innate immunity complement system Cellular localization Original Research chemistry.chemical_classification Messenger RNA Mucous Membrane Innate immune system biology Gene Expression Profiling Antibodies Monoclonal Lectin Epithelial Cells Collectins Complement system Cell biology 030104 developmental biology Liver chemistry biology.protein Immunohistochemistry lcsh:RC581-607 Protein Binding 030215 immunology |
Zdroj: | Frontiers in Immunology, Vol 9 (2018) Hansen, S W K, Aagaard, J B, Bjerrum, K B, Hejbøl, E K, Nielsen, O, Schrøder, H D, Skjoedt, K, Sørensen, A L, Graversen, J H & Henriksen, M L 2018, ' CL-L1 and CL-K1 Exhibit Widespread Tissue Distribution With High and Co-Localized Expression in Secretory Epithelia and Mucosa ', Frontiers in Immunology, vol. 9, 1757 . https://doi.org/10.3389/fimmu.2018.01757 Frontiers in Immunology |
ISSN: | 1664-3224 |
Popis: | Collectin liver 1 (CL-L1, alias collectin 10) and collectin kidney 1 (CL-K1, alias collectin 11) are oligomeric pattern recognition molecules associated with the complement system, and mutations in either of their genes may lead to deficiency and developmental defects. The two collectins are reportedly localized and synthesized in the liver, kidneys, and adrenals, and can be found in the circulation as heteromeric complexes (CL-LK), which upon binding to microbial high mannose-like glycoconjugates activates the complement system via the lectin activation pathway. The tissue distribution of homo- vs. heteromeric CL-L1 and -K1 complexes, the mechanism of heteromeric complex formation and in which tissues this occurs, is hitherto incompletely described. We have by immunohistochemistry using monoclonal antibodies addressed the precise cellular localization of the two collectins in the main human tissues. We find that the two collectins have widespread and almost identical tissue distribution with a high expression in epithelial cells in endo-/exocrine secretory tissues and mucosa. There is also accordance between localization of mRNA transcripts and detection of proteins, showing that local synthesis likely is responsible for peripheral localization and eventual formation of the CL-LK complexes. The functional implications of the high expression in endo-/exocrine secretory tissue and mucosa is unknown but might be associated with the activity of MASP-3, which has a similar pattern of expression and is known to potentiate the activity of the alternative complement activation pathway. |
Databáze: | OpenAIRE |
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