Establishment of two cell lines from embryonic tissue of the tobacco hornworm,Manduca sexta (L.)
| Autor: | Edwin P. Marks, P. E. Eide, J. M. Caldwell |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
animal structures
Cell division Cell Survival Plant Science Vacuole Cytoplasmic Granules Cell Line Freezing medicine Cells Cultured Cell Aggregation Cell Nucleus biology fungi Embryonic Tissue biology.organism_classification Cell aggregation Culture Media Cell biology Lepidoptera Cell nucleus medicine.anatomical_structure Cell culture Manduca sexta Karyotyping Vacuoles Developmental biology Cell Division Biotechnology |
| Zdroj: | In Vitro. 11:395-399 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02616376 |
| Popis: | Two cell lines were derived from primary cultures of embryonic tissue of the tobacco hornworm, Manduca sexta (L.). The cell lines were maintained on hemolymph-free synthetic insect medium. Cytogenetic and immunoligical identification of the lines were carried out. Techniques for obtaining the line and medium, subculturieng and freezing procedures for long-term storage, and the morphological and growth characteristics are described. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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