Carbon and nitrogen isotopic ratios of urine and faeces as novel nutritional biomarkers of meat and fish intake
| Autor: | Catherine J. Kneale, Annemiek M. C. P. Joosen, Gunter G. C. Kuhnle, Tamsin C. O'Connell |
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| Jazyk: | angličtina |
| Předmět: |
Adult
Male Meat 030309 nutrition & dietetics Medicine (miscellaneous) Urine Biology 01 natural sciences Feces Young Adult 03 medical and health sciences Risk Factors Animals Humans Nutritional biomarker Fish intake Food science Stable isotope ratio 2. Zero hunger Carbon Isotopes 0303 health sciences Nutritional biomarkers Cross-Over Studies Nutrition and Dietetics Nitrogen Isotopes Diet Vegetarian 010401 analytical chemistry Fishes Feeding Behavior Original Contribution Fish consumption Intervention studies 0104 chemical sciences 3. Good health Nutrition Assessment Fish Dietary assessment Biomarker (medicine) Fish Female Self Report Biomarkers |
| Zdroj: | European Journal of Nutrition |
| ISSN: | 1436-6207 1436-6215 |
| DOI: | 10.1007/s00394-012-0328-2 |
| Popis: | Purpose \ud Meat and fish consumption are associated with changes in the risk of chronic diseases. Intake is mainly assessed using self-reporting, as no true quantitative nutritional biomarker is available. The measurement of plasma fatty acids, often used as an alternative, is expensive and time-consuming. As meat and fish differ in their stable isotope ratios, δ13C and δ15N have been proposed as biomarkers. However, they have never been investigated in controlled human dietary intervention studies.\ud Objective \ud In a short-term feeding study, we investigated the suitability of δ13C and δ15N in blood, urine and faeces as biomarkers of meat and fish intake.\ud Methods \ud The dietary intervention study (n = 14) followed a randomised cross-over design with three eight-day dietary periods (meat, fish and half-meat–half-fish). In addition, 4 participants completed a vegetarian control period. At the end of each period, 24-h urine, fasting venous blood and faeces were collected and their δ13C and δ15N analysed.\ud Results \ud There was a significant difference between diets in isotope ratios in faeces and urine samples, but not in blood samples (Kruskal–Wallis test, p < 0.0001). In pairwise comparisons, δ13C and δ15N were significantly higher in urine and faecal samples following a fish diet when compared with all other diets, and significantly lower following a vegetarian diet. There was no significant difference in isotope ratio between meat and half-meat–half-fish diets for blood, urine or faecal samples.\ud Conclusions \ud The results of this study show that urinary and faecal δ13C and δ15N are suitable candidate biomarkers for short-term meat and fish intake. |
| Databáze: | OpenAIRE |
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