Anti-influenza virus activity of the ethanolic extract from Peperomia sui

Autor: Ting-Ting Jong, Chih-Hsueh Yang, Wei-Li Hsu, Chi-Luan Wen, Shih-Lan Hsu, Poa-Chun Chang, Duen-Huey Tan
Rok vydání: 2014
Předmět:
Hemagglutination
DAPI
(4′
6-diamidino-2-phenylindole)
viruses
medicine.disease_cause
Peperomia
MTT
3-(4
5-dimethylthiazol-2-yl)-2
5-diphenyltetrazolium bromide
MEM
minimal essential medium
Drug Discovery
Peperomia sui
Antiviral activity
Fluorescent Antibody Technique
Indirect
DMEM
Dulbecco׳s modified Eagle׳s medium
biology
Reverse Transcriptase Polymerase Chain Reaction
NA
neuraminidase
qRT–PCR
quantitative reverse transcription–PCR
Real-time polymerase chain reaction
CPE
cytopathic effect
Influenza A virus
EC50
half maximal effective concentration
FITC
fluorescein isothiocyanate
animal structures
Cell Survival
PBS
phosphate-buffered saline
Taiwan
Neuraminidase
CC50
50% cytotoxic concentration
Antiviral Agents
Article
Virus
Microbiology
medicine
Animals
Viability assay
Medicine
East Asian Traditional
Pharmacology
IFA
indirect immunofluorescence assay
Hemagglutination assay
MU-NANA
20-(4-Methylumbelliferyl)-a-D-N-acetylneuraminic acid
Ethanol
Plant Extracts
Fibroblasts
biology.organism_classification
Virology
Influenza A virus subtype H5N1
Nucleoprotein
IC50
half-maximum inhibitory concentration
Influenza in Birds
Influenza virus
Chickens
HA
hemagglutinin
Zdroj: Journal of Ethnopharmacology
ISSN: 0378-8741
DOI: 10.1016/j.jep.2014.05.035
Popis: Ethnopharmacological relevance Peperomia sui Lin and Lu (Peperomia sui), a well-known Taiwanese folk medicine, has a broad range of biological effects, especially in treatment of upper respiratory tract diseases. However, no previous study has explored the activity of Peperomia sui against influenza virus infections. This study was carried out to evaluate the anti-influenza virus activity and the potential virucidal effect of the ethanolic extract of Peperomia sui (PSE). Methods The anti-H6N1 avian influenza viral activity of PSE against the influenza virus A/Chicken/TW/0518/2011 (H6N1) in chicken fibroblast DF-1 cells was evaluated by cell viability assay, hemagglutination assay, neuraminidase activity assay, indirect immunofluorescence assay and quantitative RT-PCR assay. Results PSE significantly increased the viability of cells that were infected by the H6N1 virus. PSE also suppressed the synthesis of viral nucleoprotein (NP), and inhibited the growth of the virus in DF-1 cells. Further, PSE inhibited the neuraminidase activity of H6N1 virus. Conclusions The findings of this study provide important information for the exploitation and utilization of Peperomia sui in treatment of influenza infection.
Graphical abstract
Databáze: OpenAIRE
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