Diverse aminoglycoside phosphotransferase types conferring aminoglycoside resistance in Enterobacteriaceae: A single-centre study from Northeast India
| Autor: | Jayalaxmi Wangkheimayum, Mohana Bhattacharjee, Bhaskar Jyoti Das, K Melson Singha, Debadatta Dhar Chanda, Deepshikha Bhowmik, Amitabha Bhattacharjee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) antibiotic resistance Klebsiella pneumoniae 030106 microbiology Immunology lcsh:QR1-502 medicine.disease_cause Microbiology lcsh:Microbiology law.invention 03 medical and health sciences 0302 clinical medicine Plasmid Antibiotic resistance Immunology and Microbiology (miscellaneous) law aminoglycoside phosphotransferase medicine Immunology and Allergy 030212 general & internal medicine Typing Gene Escherichia coli Polymerase chain reaction Genetics aminoglycoside resistance General Immunology and Microbiology biology biology.organism_classification Enterobacteriaceae Infectious Diseases escherichia coli |
| Zdroj: | Indian Journal of Medical Microbiology, Vol 37, Iss 3, Pp 418-422 (2019) |
| ISSN: | 1998-3646 0255-0857 |
| Popis: | The present study investigates the molecular basis of aph-mediated aminoglycoside resistance and their transmission dynamics in a tertiary care hospital of Northeast India. Two hundred forty one isolates (230 Escherichia coli and 11 Klebsiella pneumoniae) were collected and screened for aminoglycoside resistance genes. Various aph types were amplified using polymerase chain reaction (PCR) assay. Plasmid incompatibilty, horizontal transferability and ERIC-PCR based typing were carried out for all the positive isolates. Among them, 67 isolates showed the presence of aph gene. Aph (3“)-IIIa and aph (3')-Via were predominant and horizontally transferable. All the plasmids were of incompatibility I1 group. Twenty-eight different haplotypes of E. coli were found harbouring aph gene types. This study was able to identify diverse aph types in a single centre and their corresponding phenotypic trait. |
| Databáze: | OpenAIRE |
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