Direct proton and natural abundance carbon-13 NMR observation of liver changes induced by ethionine
| Autor: | Ronald E. Block |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Carbon Isotopes
Fourier Analysis Proton Ethionine Chemistry Carbon-13 Biophysics Analytical chemistry Resonance Pulse sequence Cell Biology Carbon-13 NMR Biochemistry Rats Inbred F344 Rats NMR spectra database chemistry.chemical_compound Nuclear magnetic resonance Liver Proton NMR Animals Female Protons Molecular Biology |
| Zdroj: | Biochemical and Biophysical Research Communications. 108:940-947 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(82)92089-7 |
| Popis: | High resolution Fourier transform proton NMR spectra obtained from ethionine-induced fatty livers show lipid-like resonances in the 0.9–3.0 p.p.m. region in addition to the water proton resonance. Partially relaxed proton spectra obtained using the inversion recovery (180°-τ-90°) pulse sequence and spectra obtained using the spin-echo (90°-τ-180°-τ-echo) pulse sequence demonstrate that these signals are independent of the water signal. Natural abundance carbon-13 NMR spectra of fatty livers show resonances identical to those of triglycerides. It is concluded that both proton and carbon-13 NMR spectra can be used to detect the triglyceride accumulation in fatty liver development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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