A basic class I chitinase expression in winged bean is up-regulated by osmotic stress
Autor: | Yoshiko Tateishi, Yoshimi Umemura, Muneharu Esaka |
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Rok vydání: | 2001 |
Předmět: |
Signal peptide
DNA Complementary Osmotic shock DNA Plant Molecular Sequence Data Genes Plant Applied Microbiology and Biotechnology Biochemistry Gene Expression Regulation Enzymologic Analytical Chemistry chemistry.chemical_compound Gene Expression Regulation Plant Osmotic Pressure Complementary DNA Gene expression Northern blot Amino Acid Sequence RNA Messenger Molecular Biology Peptide sequence Abscisic acid biology Base Sequence Sequence Homology Amino Acid Organic Chemistry Chitinases Fabaceae General Medicine Molecular biology Up-Regulation chemistry RNA Plant Chitinase biology.protein Biotechnology |
Zdroj: | Bioscience, biotechnology, and biochemistry. 65(7) |
ISSN: | 0916-8451 |
Popis: | We isolated a cDNA for basic class I chitinase (ChitiWb1). ChitiWb1 cDNA encodes a protein that consists of 315 amino acid residues and has a signal peptide. Northern blot analysis indicated that the class I chitinase mRNA in leaves and cultured cells of winged bean was increased by treatments with NaCl, KCl, CaCl2, mannitol or saccharose, but not with abscisic acid. Thus, class I chitinase expression was shown to be up-regulated by osmotic stress. |
Databáze: | OpenAIRE |
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