Preparation and properties of monomercurated tRNA

Autor:	Magi G. Nargisyan, Edward I. Budowsky, Gulnara G. Abdurashidova
Rok vydání:	1983
Předmět:	chemistry.chemical_classification Chromatography Chemical Phenomena Organomercury Compounds Stereochemistry Aminoacylation RNA Transfer Amino Acyl Biochemistry In vitro Chemistry RNA Bacterial RNA Transfer chemistry Transfer RNA Escherichia coli Nucleotide
Zdroj:	European Journal of Biochemistry. 136:147-150
ISSN:	1432-1033 0014-2956
Popis:	Monomercurated tRNA has been prepared by exhaustive treatment of mercaptoethanol on polymercurated tRNA under non-denaturing conditions. The remained mercury atom is stable toward ultraviolet (254 nm) irradiation up to absorption of 100 quanta/nucleotide, but could be easily removed by mercaptoethanol under denaturing conditions. Monomercurated tRNA quantitatively binds to thiopropyl-Sepharose and can be completely desorbed by mercaptoethanol-containing solutions. Monomercurated tRNA is similar to the starting tRNA preparation in efficiency and specificity of aminoacylation and in factor-dependent polypeptide synthesis in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5aa8e95110cd9d1f06727899a09efd2 https://doi.org/10.1111/j.1432-1033.1983.tb07718.x Zobrazit plný text záznamu Plný text ve formátu PDF