Regulatory Patterns of Crp on Monensin Biosynthesis in Streptomyces cinnamonensis
Autor: | Yue Zhang, Jianjun Qiao, Rong-Hui Xie, Chun-Yan Lin, Jihua Wu, Guang-Rong Zhao |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Microbiology (medical)
Cyclic AMP Receptor Protein Microbiology Streptomyces 03 medical and health sciences chemistry.chemical_compound Virology Gene cluster Transcriptional regulation Gene Transcription factor lcsh:QH301-705.5 streptomyces cinnamonensis transcription factor 030304 developmental biology Regulator gene 0303 health sciences Streptomyces cinnamonensis monensin biosynthesis biology 030306 microbiology Monensin RNA sequencing biology.organism_classification chemistry Biochemistry lcsh:Biology (General) Crp transcription regulation |
Zdroj: | Microorganisms Volume 8 Issue 2 Microorganisms, Vol 8, Iss 2, p 271 (2020) |
ISSN: | 2076-2607 |
DOI: | 10.3390/microorganisms8020271 |
Popis: | Monensin, produced by Streptomyces cinnamonensis, is a polyether ionophore antibiotic widely used as a coccidiostat and a growth-promoting agent in agricultural industry. In this study, cyclic AMP receptor protein (Crp), the global transcription factor for regulation of monensin biosynthesis, was deciphered. The overexpression and antisense RNA silencing of crp revealed that Crp plays a positive role in monensin biosynthesis. RNA sequencing analysis indicated that Crp exhibited extensive regulatory effects on genes involved in both primary metabolic pathways and the monensin biosynthetic gene cluster (mon). The primary metabolic genes, including acs, pckA, accB, acdH, atoB, mutB, epi and ccr, which are pivotal in the biosynthesis of monensin precursors malonyl-CoA, methylmalonyl-CoA and ethylmalonyl-CoA, are transcriptionally upregulated by Crp. Furthermore, Crp upregulates the expression of most mon genes, including all PKS genes (monAI to monAVIII), tailoring genes (monBI-monBII-monCI, monD and monAX) and a pathway-specific regulatory gene (monRI). Enhanced precursor supply and the upregulated expression of mon cluser by Crp would allow the higher production of monensin in S. cinnamonensis. This study gives a more comprehensive understanding of the global regulator Crp and extends the knowledge of Crp regulatory mechanism in Streptomyces. |
Databáze: | OpenAIRE |
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