Heat and oxidative stress alter the expression of orexin and its related receptors in avian liver cells
| Autor: | Walter Bottje, Peter Ishola, Stephanie Khaldi, Nicholas B. Anthony, Sami Dridi, Elizabeth S. Greene, Takeshi Ohkubo |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Hot Temperature Physiology Down-Regulation Neuropeptide Coturnix Biology medicine.disease_cause Biochemistry 03 medical and health sciences Downregulation and upregulation Orexin Receptors Cell Line Tumor Orexigenic Internal medicine mental disorders medicine Animals Heat shock Receptor Molecular Biology Aldehydes Orexins Hydrogen Peroxide Orexin receptor Orexin Oxidative Stress 030104 developmental biology Endocrinology Liver nervous system Heat-Shock Response Oxidative stress medicine.drug |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 191:18-24 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2015.08.016 |
| Popis: | Orexins (A and B) or hypocretins (1 and 2) are hypothalamic orexigenic neuropeptides that are involved in the regulation of several physiological processes in mammals. Recently, orexin has been shown to activate the hypothalamic-pituitary-adrenal (HPA) stress axis and emerging evidences identify it as a stress modulator in mammals. However, the regulation of orexin system by stress itself remains unclear. Here, we investigate the effects of heat, 4-Hydroxynonenal (4-HNE) and hydrogen peroxide (H2O2) stress on the hepatic expression of orexin (ORX) and its related receptors (ORXR1/2) in avian species. Using in vivo and in vitro models, we found that heat stress significantly down-regulated ORX and ORXR1/2 mRNA and protein abundances in quail liver and LMH cells. H2O2, however, decreased ORX protein and increased ORX mRNA levels in a dose dependent manner (P |
| Databáze: | OpenAIRE |
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