Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA
| Autor: | Cuong Cao, Hywel Morgan, Dang Duong Bang, Anders Wolff, S. Birtwell, Jonas Høgberg |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
lcsh:R5-920
Fabrication Materials science 010405 organic chemistry DNA hybridization Clinical Biochemistry Nanotechnology 02 engineering and technology Photoresist autofluorescence 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence Article 0104 chemical sciences SU-8 Autofluorescence Colloidal gold gold nanoparticles solid-phase PCR amplification Thermal stability lcsh:Medicine (General) 0210 nano-technology Layer (electronics) Order of magnitude |
| Zdroj: | Diagnostics Volume 2 Issue 4 Pages 72-82 Queen's University Belfast-PURE Technical University of Denmark Orbit Cao, C, Birtwell, S W, Høgberg, J, Morgan, H, Wolff, A & Bang, D D 2012, ' Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA ', Diagnostics, vol. 2, no. 4, pp. 72-82 . Diagnostics, Vol 2, Iss 4, Pp 72-82 (2012) |
| ISSN: | 2075-4418 |
| DOI: | 10.3390/diagnostics2040072 |
| Popis: | SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material. |
| Databáze: | OpenAIRE |
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