Tissue-specific expression of a rat intestinal mucin-like peptide
| Autor: | E Cutz, D. Wang, Gongqiao Xu, L J Huan, Gordon G. Forstner, Janet F. Forstner |
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| Rok vydání: | 1992 |
| Předmět: |
Peptide Biosynthesis
Spleen Biology Biochemistry Transcription (biology) Complementary DNA medicine Animals RNA Messenger Intestinal Mucosa Molecular Biology Goblet cell Messenger RNA Mucin Mucins Nucleic Acid Hybridization RNA DNA Cell Biology Blotting Northern Submandibular gland Molecular biology Rats medicine.anatomical_structure Peptides Research Article |
| Zdroj: | Biochemical Journal. 286:335-338 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2860335 |
| Popis: | Expression of the gene for a rat intestinal mucin-like peptide (MLP) was studied by Northern-blot analyses of RNA prepared from a panel of rat tissues. Four probes (A-D) were constructed so as to span a 3.5 kb-long cDNA for rat MLP, and used for hybridization. Positive signals were obtained in intestine and colon, whereas lung, liver, stomach, submandibular gland and spleen were negative. The only transcript detected was approx. 9.5 kb in size. No mRNA splice variants were found. Hybridization in situ using probe B1, which corresponds to a cysteine-rich region near the C-terminus of MLP, confirmed that the gene for MLP is expressed by goblet cells of rat intestine and colon. |
| Databáze: | OpenAIRE |
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