A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development
Autor: | Michael A. Hauser, David M. Kurnit, Malgorzata M. Osemlak-Hanzlik, Andrzej J. Hanzlik |
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Rok vydání: | 1993 |
Předmět: |
Adult
Fragile x Transcription Genetic Molecular Sequence Data Nerve Tissue Proteins Polymerase Chain Reaction Homology (biology) Bacteriophage Fragile X Mental Retardation Protein Fetus Genetics Humans Recombination Genetic biology Base Sequence RNA-Binding Proteins DNA biology.organism_classification Bacteriophage lambda Genetic Techniques Organ Specificity Fragile X Syndrome Human fetal DNA Probes Recombination |
Zdroj: | Nature genetics. 3(1) |
ISSN: | 1061-4036 |
Popis: | To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum. |
Databáze: | OpenAIRE |
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