A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development

Autor: Michael A. Hauser, David M. Kurnit, Malgorzata M. Osemlak-Hanzlik, Andrzej J. Hanzlik
Rok vydání: 1993
Předmět:
Zdroj: Nature genetics. 3(1)
ISSN: 1061-4036
Popis: To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.
Databáze: OpenAIRE