Influence of the MACC1 Gene on Sensitivity to Chemotherapy in Human U251 Glioblastoma Cells
| Autor: | Yan Guo, Yang Hong, Chao Shang, Yun-Hui Liu, Yi-Xue Xue |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Cancer Research Epidemiology medicine.medical_treatment Apoptosis Biology Flow cytometry RNA interference Cell Line Tumor Glioma medicine Humans Gene silencing Aged Cisplatin Chemotherapy Messenger RNA medicine.diagnostic_test Brain Neoplasms Public Health Environmental and Occupational Health Middle Aged medicine.disease Molecular biology Oncology Trans-Activators Female Glioblastoma Transcription Factors medicine.drug |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 16:195-199 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2015.16.1.195 |
| Popis: | Background: This study was conducted to determine the influence of MACC1 expression on chemotherapy sensitivity in human U251 glioblastoma cells. Materials and Methods: Expression of the MACC1 gene in 49 cases of human brain glioma was determined by quantitative real-time PCR. Silencing effects of RNA interference on MACC1 was detected by Western-blotting. Flow cytometry methods and methyl thiazolyl tetrazolium assay (MTT) were used to determine the apoptosis and growth inhibitory rates of the U251 cells with MACC1 silencing. before and after treatment with cisplatin (DDP). Results: MACC1 mRNA in gliomas was up-regulated remarkably, to 158.8% of that in peri-cancerous tissues (P |
| Databáze: | OpenAIRE |
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