Dynamics of Elimination of Plasmids and Expression of VEGF121 Gene Transfected into Human Mesenchymal Stem Cells by Different Methods
| Autor: | Alexander Lavrov, S. A. Smirnikhina, N. P. Bochkov |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Vascular Endothelial Growth Factor A
Electroporation Mesenchymal stem cell Gene Expression Adipose tissue Mesenchymal Stem Cells General Medicine Transfection Biology Lipids Molecular biology General Biochemistry Genetics and Molecular Biology Plasmid Adipose Tissue DNA methylation Gene expression Humans Gene Cells Cultured Plasmids |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 151:121-125 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-011-1272-5 |
| Popis: | We compared two methods of transfection (lipofection and electroporation) with plasmid containing VEGF121 gene in four cultures of mesenchymal stem cells from the human adipose tissue. The efficacy of transfection after 1 day, the dynamics of plasmid elimination after 3, 6, 9 days, and expression of the target gene were evaluated. Transfection by both methods failed in one of 4 cultures. Analysis of the plasmid elimination dynamics showed that the content of plasmids introduced by both methods decreased by 30-69% in all cultures by day 3 and then remained unchanged from day 3 to day 9. The expression of the target gene did not correlate with the content of plasmids in cells and varied by 2-10 times in control cells and cells transfected by both methods. Fluctuation of VEGF121 expression was not related to methylation. |
| Databáze: | OpenAIRE |
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