Acceleration of Small Intestine Development and Remodeling of the Microbiome Following Hyaluronan 35 kDa Treatment in Neonatal Mice
| Autor: | Cristina Lupu, Robert Silasi, Girija Regmi, Ravi S. Keshari, Kathryn Burge, Misty Good, Florea Lupu, Majoi A. Trammell, Hala Chaaban, Steven J. McElroy, Jeffrey Eckert, David W. Dyer |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Small Mice 0302 clinical medicine Intestine Small 2.1 Biological and endogenous factors TX341-641 Intestinal Mucosa Hyaluronic Acid Pediatric Nutrition and Dietetics Cell Differentiation Intestine Intestines medicine.anatomical_structure Goblet Cells Pediatric Research Initiative Paneth Cells 1.1 Normal biological development and functioning Crypt Biology digestive system Article Microbiology hyaluronan 03 medical and health sciences Food Sciences Downregulation and upregulation medicine Animals Microbiome preterm infants Nutrition Cell Proliferation Goblet cell necrotizing enterocolitis human milk bioactive factors Nutrition. Foods and food supply Cell growth Lachnospiraceae Perinatal Period - Conditions Originating in Perinatal Period Newborn Small intestine Gastrointestinal Microbiome intestinal barrier 030104 developmental biology Animals Newborn Dietary Supplements Colostrum Digestive Diseases prebiotics 030217 neurology & neurosurgery Food Science |
| Zdroj: | Nutrients Nutrients, vol 13, iss 6 Nutrients, Vol 13, Iss 2030, p 2030 (2021) Volume 13 Issue 6 |
| ISSN: | 2072-6643 |
| Popis: | The beneficial effects of human milk suppressing the development of intestinal pathologies such as necrotizing enterocolitis in preterm infants are widely known. Human milk (HM) is rich in a multitude of bioactive factors that play major roles in promoting postnatal maturation, differentiation, and the development of the microbiome. Previous studies showed that HM is rich in hyaluronan (HA) especially in colostrum and early milk. This study aims to determine the role of HA 35 KDa, a HM HA mimic, on intestinal proliferation, differentiation, and the development of the intestinal microbiome. We show that oral HA 35 KDa supplementation for 7 days in mouse pups leads to increased villus length and crypt depth, and increased goblet and Paneth cells, compared to controls. We also show that HA 35 KDa leads to an increased predominance of Clostridiales Ruminococcaceae, Lactobacillales Lactobacillaceae, and Clostridiales Lachnospiraceae. In seeking the mechanisms involved in the changes, bulk RNA seq was performed on samples from the terminal ileum and identified upregulation in several genes essential for cellular growth, proliferation, and survival. Taken together, this study shows that HA 35 KDa supplemented to mouse pups promotes intestinal epithelial cell proliferation, as well as the development of Paneth cells and goblet cell subsets. HA 35 KDa also impacted the intestinal microbiota the implications of these responses need to be determined. |
| Databáze: | OpenAIRE |
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