Copper metallothionein in patients with hepatic copper overload
| Autor: | P. E. Hunziker, I. Sternlieb |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male Gene isoform medicine.medical_specialty Adolescent Clinical Biochemistry chemistry.chemical_element Cholestatic cirrhosis Biochemistry Hepatic copper Cytosol Hepatolenticular Degeneration Biliary Atresia Biliary atresia Internal medicine medicine Humans Metallothionein Child Liver Cirrhosis Biliary business.industry General Medicine medicine.disease Copper Wilson's disease Endocrinology Liver chemistry Child Preschool Female business |
| Zdroj: | European Journal of Clinical Investigation. 21:466-471 |
| ISSN: | 1365-2362 0014-2972 |
| DOI: | 10.1111/j.1365-2362.1991.tb01396.x |
| Popis: | We studied the Cu-MT present in the hepatic cytosol obtained from 7 patients suffering from conditions associated with hepatic Cu overload (Wilson's disease, biliary atresia, familial cholestatic cirrhosis). Since chromatographic methods appropriate for the isolation of Zn- and Cd-MT were unsuitable for Cu-MT, we developed an indirect procedure for the estimation and resolution of the latter. This procedure involved the preparation of apo-MT and its reconstitution to holo-MT with Zn or Cd. Three predominant isoforms of MT were present in all specimens. Our results indicate that at most 36 +/- 5% of the Cu present in the 10 kDa fraction of cytosol is bound to MT in the liver of patients with hepatic copper overload. |
| Databáze: | OpenAIRE |
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