Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)
| Autor: | Andony P. Melathopoulos, Emily A Carlson, Priyadarshini Chakrabarti, Ramesh R. Sagili, Hannah M Lucas |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Insecticides Time Factors Pyridines Apoptosis medicine.disease_cause 01 natural sciences Toxicology chemistry.chemical_compound 4-Butyrolactone Pollinator Pollination Sulfoxaflor 0303 health sciences Multidisciplinary Cell Death biology Caspase 3 Eukaryota Insect physiology Agriculture Bees Insects Cell Processes Insect Proteins Medicine Honey Bees Agrochemicals Research Article Arthropoda Cell Survival Science Insect Physiology 03 medical and health sciences medicine Animals Animal Physiology Pesticides Sugar 030304 developmental biology Invertebrate Physiology Sulfur Compounds business.industry Organisms Pest control Biology and Life Sciences Cell Biology Honey bee Pesticide biology.organism_classification Invertebrates Hymenoptera Oxidative Stress 010602 entomology chemistry Pest Control business Zoology Entomology Oxidative stress |
| Zdroj: | PLoS ONE, Vol 15, Iss 5, p e0233033 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0233033 |
| Popis: | Pesticide exposures can have detrimental impacts on bee pollinators, ranging from immediate mortality to sub-lethal impacts. Flupyradifurone is the active ingredient in Sivanto™ and sulfoxaflor is the active ingredient in Transform®. They are both relatively new insecticides developed with an intent to reduce negative effects on bees, when applied to bee-attractive crops. With the growing concern regarding pollinator health and pollinator declines, it is important to have a better understanding of any potential negative impacts, especially sub-lethal, of these pesticides on bees. This study reports novel findings regarding physiological stress experienced by bees exposed to field application rates of these two insecticides via a Potter Tower sprayer. Two contact exposure experiments were conducted-a shorter 6-hour study and a longer 10-day study. Honey bee mortality, sugar syrup and water consumption, and physiological responses (oxidative stress and apoptotic protein assays) were assessed in bees exposed to Sivanto™ and Transform®, and compared to bees in control group. For the longer, 10-day contact exposure experiment, only the Sivanto™ group was compared to the control group, as high mortality recorded in the sulfoxaflor treatment group during the shorter contact exposure experiment, made the latter group unfeasible to test in the longer 10-days experiment. In both the studies, sugar syrup and water consumptions were significantly different between treatment groups and controls. The highest mortality was observed in Transform® exposed bees, followed by the Sivanto™ exposed bees. Estimates of reactive oxygen/nitrogen species indicated significantly elevated oxidative stress in both pesticide treatment groups, when compared to controls. Caspase-3 protein assays, an indicator of onset of apoptosis, was also significantly higher in the pesticide treatment groups. These differences were largely driven by post exposure duration, indicating sub-lethal impacts. Further, our findings also emphasize the need to revisit contact exposure impacts of Sivanto™, given the sub-lethal impacts and mortality observed in our long-term (10-day) contact exposure experiment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|