Standardized methods for acute and semichronic toxicity tests with the copepod Acartia tonsa
| Autor: | Gessica Gorbi, F. Savorelli, Ines Lacchetti, Elisabetta Giacco, Monica Cigar, Isabella Buttino, Olga Faraponova, Tristano Leoni, Marion Invidia, Sandra Sei, Ermelinda Prato |
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| Rok vydání: | 2012 |
| Předmět: |
Health
Toxicology and Mutagenesis ved/biology.organism_classification_rank.species Zooplankton Copepoda Toxicology chemistry.chemical_compound Nickel Toxicity Tests Acute Animals Environmental Chemistry Ecotoxicology Bioassay Reference standards Acartia tonsa Life Cycle Stages biology ved/biology Reproduction Toxicity Tests Subchronic Reproducibility of Results Reference Standards biology.organism_classification chemistry Toxicity Copepod Toxicant |
| Zdroj: | Environmental Toxicology and Chemistry. 31:2023-2028 |
| ISSN: | 0730-7268 |
| DOI: | 10.1002/etc.1909 |
| Popis: | The availability of standardized protocols for both organism culture and bioassay with ecologically relevant species is of great concern in ecotoxicology. Acartia tonsa represents an important, often dominant, member of zooplankton communities and meets all the practical criteria suggested for model species. New standardized procedures for laboratory culturing of the copepod A. tonsa and standardized methods for acute (24- and 48-h) and semichronic (7-d, static-renewal) toxicity tests with the nauplius stage are described. In both cases, eggs are the starting stage, and nauplius immobilization is the endpoint. The methods were the object of an intercomparison test involving nine laboratories, and nickel was the reference toxicant. Relative reproducibility was 24, 25, and 34% for 24-h, 48-h, and 7-d tests, respectively. |
| Databáze: | OpenAIRE |
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