Demulsifying properties of extracellular products and cells of Pseudomonas aeruginosa MSJ isolated from petroleum-contaminated soil
| Autor: | J.C.C. Barcelos, Andrea de Souza Monteiro, Joana O. P. A. Coutinho, P.M. Moraes, M.P.S. Silva, Ezequias P. Siqueira, Vera Lúcia dos Santos |
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| Rok vydání: | 2013 |
| Předmět: |
Environmental Engineering
Demulsification Bioengineering Industrial Oils medicine.disease_cause Diesel fuel Species Specificity medicine Extracellular Soil Pollutants Waste Management and Disposal Soil Microbiology Water-in-oil emulsions Chromatography Strain (chemistry) Renewable Energy Sustainability and the Environment Pseudomonas aeruginosa Chemistry Oil-in-water emulsions General Medicine Demulsifier Soil contamination Biodegradation Environmental Emulsion Emulsions Soil microbiology |
| Zdroj: | Bioresource Technology. 128:646-654 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2012.09.137 |
| Popis: | A strain of Pseudomonas aeruginosa isolated from a site contaminated with refined oil products exhibited demulsification capabilities against Tween 80–Span 80 stabilized oil-in-water (O/W), Tween 80-stabilized water-in-oil (W/O) model emulsions (kerosene–water), and an industrial emulsion (Daido Dairoll PA-5A). GC–MS analysis confirmed the presence of fatty acids and carbohydrates in the extracellular biodemulsifier. The demulsifying activity of cells and culture supernatants was favored by growth in media containing 1% diesel oil. There was a correlation between culture age, de-emulsification and cellular hydrophobicity, and highest activities were observed for cells and supernatants from 96-h cultures. Activity increased with addition of up to 60mg cells or 300μL supernatant to emulsions. The activity was relatively stable at 20–40°C and to freezing, but was reduced by 69% by washing the cells with chloroform–methanol-water. This demulsifier has potential for application in biotreatment of emulsified oily wastewaters to promote recovery and/or degradation of oil. |
| Databáze: | OpenAIRE |
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