Novel Electrochemically Switchable, Flexible, Microporous Cloth that Selectively Captures, Releases, and Concentrates Intact Extracellular Vesicles
| Autor: | Cherie Blenkiron, Lawrence W. Chamley, Clive W. Evans, David E. Williams, Diane Brewster, Yohanes Nursalim, Valentina Lucarelli, David Barker, Alireza Akbarinejad, Saman Sabet, Colin L. Hisey, Vanessa Chang, Jadranka Travas-Sejdic, Jesna Ashraf |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Polymers Tetraspanin 30 Aptamer 010401 analytical chemistry Electrochemical Techniques Microporous material Aptamers Nucleotide Bridged Bicyclo Compounds Heterocyclic 01 natural sciences Extracellular vesicles Electrospinning Cell Line 0104 chemical sciences Extracellular Vesicles Chemical engineering PEDOT:PSS MCF-7 Cells Humans General Materials Science Gold Porosity Sulfur |
| Zdroj: | ACS Applied Materials & Interfaces. 12:39005-39013 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | There is a significant and growing research interest in the isolation of extracellular vesicles (EVs) from large volumes of biological samples and their subsequent concentration into clean and small volumes of buffers, especially for applications in medical diagnostics. Materials that are easily incorporated into simple sampling devices and which allow the release of EVs without the need for auxiliary and hence contaminating reagents are particularly in demand. Herein, we report on the design and fabrication of a flexible, microporous, electrochemically switchable cloth that addresses the key challenges in diagnostic applications of EVs. We demonstrate the utility of our electrochemically switchable substrate for the fast, selective, nondestructive, and efficient capture and subsequent release of EVs. The substrate consists of an electrospun cloth, infused with a conducting polymer and decorated with gold particles. Utilizing gold-sulfur covalent bonding, the electrospun substrates may be functionalized with SH-terminated aptamer probes selective to EV surface proteins. We demonstrate that EVs derived from primary human dermal fibroblast (HDFa) and breast cancer (MCF-7) cell lines are selectively captured with low nonspecific adsorption using an aptamer specific to the CD63 protein expressed on the EV membranes. The specific aptamer-EV interactions enable easy removal of the nonspecifically bound material through washing steps. The conducting polymer component of the cloth provides a means for efficient (92%) and fast (5 min) electrochemical release of clean and intact captured EVs by cathodic cleavage of the Au-S bond. We demonstrate successful capture of diluted EVs from a large volume sample and their release into a small volume of clean phosphate-buffered saline buffer. The developed cloth can easily be incorporated into different designs for separation systems and would be adaptable to other biological entities including cells and other EVs. Furthermore, the capture/release capability holds great promise for liquid biopsies if used to targeted disease-specific markers. |
| Databáze: | OpenAIRE |
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